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Protocols

LACS specifications

Information


Unique identifier OMICS_06816
Name LACS
Alternative name Linear Analysis of Chemical Shifts
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for Linear Analysis of Chemical Shifts

LACS citations

 (4)
call_split

PINE SPARKY.2 for automated NMR based protein structure research

2017
Bioinformatics
PMCID: 5925765
PMID: 29281006
DOI: 10.1093/bioinformatics/btx785
call_split See protocol

[…] s the status file from the URL associated with the Key, and the PINE web server updates the status of the PINE job in the status file. Predictions of secondary structures (PECAN), referencing errors (LACS), hydrophobicities (PACSY), torsion angles and flexibilities (TALOS-N), and 3 D structures (CS-ROSETTA) are executed sequentially by BASH and PYTHON scripts based on the chemical shifts with the […]

library_books

Integrative NMR for biomolecular research

2016
J Biomol NMR
PMCID: 4861749
PMID: 27023095
DOI: 10.1007/s10858-016-0029-x

[…] Linear Analysis of Chemical Shifts (LACS) is supported by NMRFAM-SPARKY (two-letter-code lv); LACS detects and corrects errors in chemical shift referencing (Wang et al. ). ARECA (Assessment of the RE […]

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An Amphipathic α Helix Controls Multiple Roles of Brome Mosaic Virus Protein 1a in RNA Replication Complex Assembly and Function

2009
PLoS Pathog
PMCID: 2654722
PMID: 19325881
DOI: 10.1371/journal.ppat.1000351
call_split See protocol

[…] equences are available from the National Magnetic Resonance Facility at Madison. Automatically generated peak lists from HIFI were used as input to the automated assignment package suite (PISTACHIO , LACS , and PECAN . 3D 15N-edited 1H-1H NOESY and 3D 13C-edited 1H-1H NOESY spectra were used as input for the ATNOS/CANDID/CYANA suite of programs ,,. The Protein Structure Validation Software suite o […]

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The Center for Eukaryotic Structural Genomics

2009
J Struct Funct Genomics
PMCID: 2705709
PMID: 19130299
DOI: 10.1007/s10969-008-9057-4
call_split See protocol

[…] uns yield reliable backbone assignments for more than 90% of the amino acid residues and reliable side chain assignments with greater than 75% completeness. The assigned data are then run through the LACS [] (Linear Analysis of Chemical Shifts) software, which corrects possible referencing problems and identifies assignment outliers. The software package PECAN [] (Protein Energetic Conformational […]


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LACS institution(s)
National Magnetics Resonance Facility at Madison, Biochemistry Department, University of Wisconsin-Madison, Madison, WI, USA

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