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LAMP Designer specifications

Information


Unique identifier OMICS_33526
Name LAMP Designer
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Version 1.15
Stability Stable
Registration required Yes
Maintained Yes

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No version available

LAMP Designer citations

 (24)
library_books

Highly sensitive detection of Salmonella typhi in clinical blood samples by magnetic nanoparticle based enrichment and in situ measurement of isothermal amplification of nucleic acids

2018
PLoS One
PMCID: 5874042
PMID: 29590194
DOI: 10.1371/journal.pone.0194817

[…] hates and ds-DNA binding dye (Optigene, UK). The FIP, BIP, F3 and B3 primers for the STY2879 gene were obtained from Fan et al.[]. The loop primers LF and LB were designed using a commercial software LAMP designer software version 1.10 (Optigene, UK). […]

library_books

Optimization of a magnetic capture RT LAMP assay for fast and real time detection of potato virus Y and differentiation of N and O serotypes

2017
Arch Virol
PMCID: 5799334
PMID: 29119360
DOI: 10.1007/s00705-017-3635-3

[…] Different primer sets were tested in this study, including the N set developed by Nie [], the Y5 set developed by Przewodowska et al. [], and a new Y4 primer set that we designed using LAMP Designer software (Premier Biosoft) as described by Przewodowska et al. [] (Table ). All primer sets consisted of four (N set) or six (Y4 and Y5 sets) primers and targeted different regions of th […]

library_books

Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop mediated isothermal amplification

2017
Sci Rep
PMCID: 5647432
PMID: 29044149
DOI: 10.1038/s41598-017-13836-9

[…] the E protein. The ZIKV sequences available in GenBank were aligned using CLUSTALX to identify conserved regions. A consensus sequence for a region in the E gene was used to design LAMP primers using LAMP Designer (Optigene; http://www.optigene.co.uk/lamp-designer/). Primers specific for Asian genotype viruses were designed first, and then African genotype-specific primers were designed by adaptin […]

call_split

Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII (A/I)I phytoplasma in Mexico

2017
Sci Rep
PMCID: 5430490
PMID: 28424530
DOI: 10.1038/s41598-017-00895-1
call_split See protocol

[…] Amplification primers (Supplementary Table ) for LAMP were designed using LAMP Designer v. 1.12 (Premier Biosoft, Palo Alto, CA). LAMP conditions were as described for calcein detection, and a LAMP temperature of 63 °C (1 hour assay time) was used. Alternatively, the same L […]

library_books

A smartphone based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

2017
Sci Rep
PMCID: 5357913
PMID: 28317856
DOI: 10.1038/srep44778

[…] 7 Zika virus isolates from the Asian/Latin American outbreak lineage. Alignment was performed with Clustal X2 software using default parameters, and scanned visually for regions of high conservation. LAMP Designer software (Premier Biosoft) was used to identify candidate primer sets for regions of high conservation (typically using an input range of 300–500 bases identified from software), using t […]

library_books

Quantitative molecular diagnostic assays of grain washes for Claviceps purpurea are correlated with visual determinations of ergot contamination

2017
PLoS One
PMCID: 5336299
PMID: 28257512
DOI: 10.1371/journal.pone.0173495

[…] Amplification primers for LAMP () that targeted cpn60 of C. purpurea were designed using LAMP Designer v. 1.12 (Premier Biosoft, Palo Alto, CA). LAMP conditions were as described for detection using calcein []. The same primers were also used for amplification and detection using Isotherm […]

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LAMP Designer institution(s)
PREMIER Biosoft International, Palo Alto, CA, USA

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