A genome assembly constructed by a hybrid assembly integrating Sanger-sequencing data from TAC/BAC clones and shotgun approaches with Illumina sequencing data to 40x genome coverage. A total of 132 scaffolds covering 232 Mbp were aligned to the six Lotus japonicus chromosomes, which was increased from 195 Mbp in build 2.5. In addition, 23,572 contigs, corresponding to 162 Mbp of the genome, were assigned to a virtual chromosome 0. The N50 values for anchored and unanchored contigs were 118,094 and 13,969 bp, respectively. To annotate genes and evaluate the gene space coverage, gene models were generated based on ab initio predictions and on RNA-seq data.
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan; Graduate School of Human Development and Environment, Kobe University, Kobe, Japan; Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan; Institute of Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan; Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan