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Protocols

m2g specifications

Information


Unique identifier OMICS_23035
Name m2g
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Not allowed
Maintained Yes

Taxon


  • Primates
    • Homo sapiens

Subtool


  • MDMG

Maintainer


  • person_outline Joshua Vogelstein

Publication for m2g

m2g citations

 (18)
library_books

Identification of tRNA nucleoside modification genes critical for stress response and development in rice and Arabidopsis

2017
BMC Plant Biol
PMCID: 5740945
PMID: 29268705
DOI: 10.1186/s12870-017-1206-0

[…] te of the two groups of 5-methylpyrimidine MTases. On the contrary, group II and group III MTases were responsible for methylpurine modifications: Trm3p, Trm8p and Trm11p corresponding to Gm, m7G and m2G in group II, and Trm5p, Trm10p and Trm1p corresponding to m2 2G and m1G methylations in group III. In between group II and III we found Trm61p and Trm13p relatives for Am and m1A methylations (Fig […]

library_books

A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity

2017
MBio
PMCID: 5347347
PMID: 28246363
DOI: 10.1128/mBio.02238-16

[…] C. crescentus and E. coli strains were imaged on M2G-1.5% agarose pads. Phase-contrast and fluorescence microscopy images were obtained using a Leica DM6000 B microscope with an HCX PL APO 100×/1.40 oil PH3 CS objective, Hamamatsu electron-multiplyi […]

library_books

Caulobacter crescentus CdnL is a non essential RNA polymerase binding protein whose depletion impairs normal growth and rRNA transcription

2017
Sci Rep
PMCID: 5324124
PMID: 28233804
DOI: 10.1038/srep43240

[…] Cells expressing Pvan-cdnLCc or Pvan-cdnLCc-flag were grown in 10 ml M2G medium with appropriate antibiotics and 0.5 mM vanillate (M2G-vanillate) overnight at 30 °C. 1 ml of this was innoculated into fresh 50 ml M2G-vanillate and grown to an OD660 = 0.3–0.4. Two 4 ml a […]

library_books

Structural and functional analyses of the archaeal tRNA m2G/m22G10 methyltransferase aTrm11 provide mechanistic insights into site specificity of a tRNA methyltransferase that contains common RNA binding modules

2016
Nucleic Acids Res
PMCID: 5291279
PMID: 27325738
DOI: 10.1093/nar/gkw561

[…] DALI server (). The most similar structure identified (i.e. the structure with the highest Z-score), was archaeal Trm14 from Pyrococcus furiosus (Pfu) (Figure and ), which catalyzes the formation of m2G6 in archaeal tRNA species (). Furthermore, the structure of NFLD and THUMP domain in TkoTrm11 is homologous to that of the NFLD and THUMP domain in other two tRNA-modifying enzymes ThiI () and CDA […]

call_split

A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues

2016
Nat Commun
PMCID: 4853435
PMID: 27117914
DOI: 10.1038/ncomms11454
call_split See protocol

[…] Cell cultures were grown overnight in M2G to an OD600 of 0.2–0.4, then immobilized onto a 1.5% agarose/M2G pad. Samples were imaged on a Leica DM 6000 B microscope with a HCX 100X PH3 1.40 NA 0.1 objective, Hamamatsu EM-CCD C9100 camera, […]

library_books

Functional dichotomy and distinct nanoscale assemblies of a cell cycle controlled bipolar zinc finger regulator

2016
eLife
PMCID: 5182063
PMID: 28008851
DOI: 10.7554/eLife.18647.019

[…] PYE or M2G cultivated cells in exponential growth phase were immobilized using a thin layer of 1% agarose. Fluorescence and DIC images were taken with an Alpha Plan-Apochromatic 100x/1.46 DIC(UV) VIS-IR oil […]

Citations

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m2g institution(s)
Johns Hopkins University, Baltimore, MD, USA; Child Mind Institute, New York, NY, USA

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