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Localization of macromolecules in cryo-electron tomograms is one of the key procedures to unravel structural features of imaged macromolecules. Positions of localized molecules are further used for structural analysis by single particle procedures such as fine alignment, averaging and classification. Accurate localization can be furthermore used to generate molecular atlases of whole cells.
(Hrabe., 2015) Localize.pytom: a modern webserver for cryo-electron tomography. Nucleic Acids Res.