MacVector protocols

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MacVector specifications

Information


Unique identifier OMICS_08914
Name MacVector
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS
License Commercial
Computer skills Medium
Version 13.5
Stability Stable
Maintained Yes

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Publication for MacVector

MacVector in pipelines

 (71)
2018
PMCID: 5770437
PMID: 29339766
DOI: 10.1038/s41598-018-19192-6

[…] of dmso., the amino acid sequences of the enzymes were collected from the ncbi genebank. the sequence alignment was carried out using the online multiple sequence alignment tools clustal omega and macvector. the secondary structure analysis was carried out using the online tool stride. the alignment figure was created using aline. the superimposition of the crystal structures and the figures […]

2018
PMCID: 5822904
PMID: 29581960
DOI: 10.1155/2018/6897032

[…] in corresponding reactions for the wheat α-tubulin gene. the relative expression was estimated by employing the 2−∆∆ct method []., the rt-pcr primers used in the present study were designed with macvector 11 (macvector, nc) and are listed in table . the product length ranged from 150–300 bp., genes were functionally annotated through homologous search of the ncbi nonredundant (nr) protein, […]

2017
PMCID: 5374973
PMID: 28367378
DOI: 10.7717/peerj.3148

[…] a total of 214 contigs (longest length: 16,445 bp, average length: 2,271 bp, n50: 3,254 bp) with total length 486,008 bp were generated. next, we remapped the 214 contigs onto the draft mt genome in macvector, and corrected 168 sequencing mistakes generated by 454 gs flx titanium platform (especially in a/t enriched regions). finally, the complete mt genome sequence of s. suchowensis […]

2017
PMCID: 5480776
PMID: 28501701
DOI: 10.1016/j.dnarep.2017.04.005

[…] was calculated between a600 values of 0.08–0.16., plasmid × chromosome recombination assays were carried out as described ., primer design, and dna and protein sequence analysis were performed using macvector (macvector inc.). predicted hydrophobicity indices were calculated using the kyte-doolittle scale . sequence alignments were performed using clustalw (gonnet series, open gap penalty […]

2017
PMCID: 5560746
PMID: 28787449
DOI: 10.1371/journal.ppat.1006538

[…] clones were selected for each sample from each of five macaques, and the complete envelope gene was sequenced., full length siv envelope sequences were aligned using the clustal w program from the macvector 15.1.1 software suite (macvector inc, apex nc usa) with minor manual adjustments. phylogenetic trees were constructed using the neighbor-joining method using mega 6.06 software suite. […]


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MacVector in publications

 (529)
PMCID: 5941075
PMID: 29739903
DOI: 10.1128/mBio.00769-18

[…] (genotype 1a, burma strain) was used as the reference strain for numbering. a clustalw multiple-sequence alignment was performed on regions containing the putative subgenomic promoter (sgp), using macvector software version 15.1.5., to construct lentiviral constructs encoding orf1 of kernow c1/p6 (genbank accession number jq679013), the kernow c1/p6 orf1 cdna was amplified by pcr […]

PMCID: 5940840
PMID: 29739933
DOI: 10.1038/s41467-018-04128-5

[…] or sanger sequencing, using the primers and methods described in piscuoglio et al.. sequencing reactions were performed in triplicate, and both the forward and reverse strands were analyzed using macvector software., for western blotting, we employed antibodies against hras (santa cruz biotechnology sc520, rabbit, 1:500), αsma (abcam ab5694, rabbit, 1:500), ck5 (thermofisher ma5-17057, mouse, […]

PMCID: 5934371
PMID: 29725036
DOI: 10.1038/s41598-018-25116-1

[…] instructions; ≤ 2 µg of rna was used per cdna synthesis. pcr was performed using taq dna polymerase (qiagen, manchester, uk) according to the manufacturer’s instructions. primers were designed using macvector7.2 (table ). for controls, rna was extracted from cell suspensions of dissociated human ovarian tissue and rodent skeletal muscle using the protocols described above. a positive control […]

PMCID: 5908835
PMID: 29674690
DOI: 10.1038/s41598-018-24697-1

[…] was used as reference. for kcnj6, relevant sequence information was retrieved at ensembl (human genome, grch38.p10); transcript features were then locally mapped on their genomic locus, via macvector. candidate primer couples, aimed at detecting coding cdna, were obtained with primer. to prevent amplification from genome, we imposed that at least one primer spans an exon-exon boundary. […]

PMCID: 5919676
PMID: 29659623
DOI: 10.1371/journal.ppat.1007003

[…] into pcdna3.1/v5-his-topo vector using the pcdna3.1 directional topo expression kit (invitrogen)., coreceptor clones were isolated, sanger sequenced, and analyzed using the clustalw algorithm with macvector 15.5.0., because mus and gsn cdna was not available, we amplified the nine exons of cd4 from gdna in four amplicons using primers flanking the exons, which were designed based on sequences […]


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MacVector institution(s)
Oxford Molecular Group, Campbell, CA, USA

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