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|Alternative name||Microarray Analysis of Differential Splicing|
|Interface||Command line interface|
|Restrictions to use||None|
|Operating system||Unix/Linux, Mac OS, Windows|
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Publication for Microarray Analysis of Differential Splicing
The conserved ubiquitin like protein Hub1 plays a critical role in splicing in human cells
[…] >0.03 significant). For initial analysis CEL files were processed using AltAnalyze software (version 2.0) with core probe set filtering using DABG (detected above background P-value cutoff 0.05) and microarray analysis of differential splicing (MiDAS exon analysis parameters P-value cutoff 0.05) (; ). The resulting splice index corresponds to differential exon intensity levels in Hub1 knockdown s […]
Posttranscriptional regulation by RNA binding proteins during epithelial to mesenchymal transition
[…] lara, CA, USA) were used to identify changes in splicing profiles after ectopic overexpression of ESRP1 in MDA-MB231 mesenchymal cells and after knockdown of ESRP1 and ESRP2 in PNT2 epithelial cells. Microarray analysis of differential splicing plus (MADS+) computational pipeline was used to identify ESRP-regulated changes in splicing. HJAY arrays consist of eight tiled probes per probe set that t […]
CD28 Costimulation Regulates Genome Wide Effects on Alternative Splicing
[…] -corrected probe intensity to the average expression level of all probes for that transcript. Different splicing indices between treatment conditions would indicate differential splicing. We used the microarray analysis of differential splicing (MADS) and microarray detection of alternative splicing (MIDAS) software to calculate probability values of differential splicing for each probeset . For s […]
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