Mascot Percolator specifications

Information


Unique identifier OMICS_24950
Name Mascot Percolator
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
Computer skills Advanced
Version 2.0
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Jyoti Choudhary <>

Publications for Mascot Percolator

Mascot Percolator in publications

 (16)
PMCID: 5846740
PMID: 29529074
DOI: 10.1371/journal.pone.0193462

[…] the spectra and its target was 3e6 for full ms and 1e5 for ms 2. the m/z scan range was 350~2000 da for ms scans and 100~1800 da for ms 2 scans., protein identification was analyzed with the cutoff: mascot percolator [] q-value < = 0.01 and protein quantification was applied using bgi's iquant []. proteins with a 1.2 fold change and a q-value of less than 0.05 were determined […]

PMCID: 5877528
PMID: 29495482
DOI: 10.3390/ijms19030667

[…] a confidence interval ≥95% according to the mascot probability scores. , protein quantitation was performed using iquant software (v2.2.1, bgi-shenzhen, shenzhen, china) [], which integrates the mascot percolator [] algorithm. simultaneously, a false discovery rate (fdr) analysis was performed using the strategy of picked protein fdr []. low confidence of peptides with a global fdr ≥1% […]

PMCID: 5790011
PMID: 29382883
DOI: 10.1038/s41598-018-20065-1

[…] identified. each identified positive protein contained at least one unique peptide. iquant software was used to quantitatively analyze the labeled peptides with isobaric tags. it integrates mascot percolator and advanced statistical algorithms to process the ms/ms signals generated from the peptides labeled by isobaric tags. any protein changed with a 1.2-fold difference and a corrected […]

PMCID: 5705591
PMID: 29184116
DOI: 10.1038/s41598-017-16718-2

[…] 0.01. at least one unique peptide was required for each identified protein., an automated software called iquant for quantitatively analyzing the labeled peptides with isobaric tags. it integrates mascot percolator and advanced statistical algorithms to process the ms/ms signals generated from the peptides labeled by isobaric tags. the mean of all labeled samples was used as a reference […]

PMCID: 5566445
PMID: 28827639
DOI: 10.1038/s41598-017-09546-x

[…] using the protein abundance for any given protein. we used p-value < 0.05 and only those with fold changes >1.5 were considered as differentially expressed proteins. in iquant analysis, the mascot percolator algorithm methods was combined and protein-level fdr < = 0.01 was used to decrease the false positive rate. the statistical analysis refers to permutation tests […]


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Mascot Percolator institution(s)
Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Hinxton,UK; Science for Life Laboratory, School of Biotechnology, Royal Institute of Technology (KTH), Solna, Sweden
Mascot Percolator funding source(s)
Supported by the Wellcome Trust [079643/Z/06/Z].

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