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Protocols
Mascot Server specifications
Information
Unique identifier | OMICS_02398 |
---|---|
Name | Mascot Server |
Alternative name | Mascot search engine |
Interface | Web user interface, Application programming interface |
Restrictions to use | License purchase required |
Computer skills | Basic |
Version | 2.6.1 |
Stability | Stable |
Maintained | Yes |
Subtool
- Mascot Daemon
Maintainers
- person_outline Gonçalo Abecasis
- person_outline Christian Fuchsberger
- person_outline Mascot Team
Publication for Mascot Server
Probability-based protein identification by searching sequence databases using mass spectrometry data.
Mascot Server citations
(2791)Smooth muscle like Ca2+ regulation of actin–myosin interaction in adult jellyfish striated muscle
[…] MS/MS spectra of the major peptide ions observed were subjected, respectively to peptide mass fingerprinting (PMF) and MS/MS ions search (MIS), using the transcrisptome database registered on a local Mascot server (Version 2.2.07, Matrix Science). The following parameters were used: allowance of one (trypsin) or no (lysylendopeptidase) missed cleavage; mass tolerance, ±0.4 Da; peptide charge, 1+; […]
Possible role of the Nipah virus V protein in the regulation of the interferon beta induction by interacting with UBX domain containing protein1
[…] ptides were extracted with 5% (v/v) formic acid and acetonitrile, and analyzed with nanoscale liquid chromatography electrospray tandem mass spectrometry. The detected peptides were analyzed with the Mascot search engine. […]
Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes
[…] nergy of -35 eV, an isolation width of m/z 1.0, an activation Q value of 0.250, and a time of 30 ms were used. Peptide MS/MS spectra were searched against UniProt and NCBI pig protein databases using Mascot server (v.2.2.04, Matrix Science Inc., MA, USA) with in-house build mucin database. The amino acid sequences of the Sus scrofa MUC5AC and MUC5B homologs were previously only partial []. They we […]
Liquid Biopsy of Vitreous Reveals an Abundant Vesicle Population Consistent With the Size and Morphology of Exosomes
[…] The 15 most abundant ions were selected for MS/MS. Data were searched using the Mascot search engine with the SwissProt Human (forward and reverse appended with common contaminant proteins) database set to carbamidomethyl (C) fixed modification. Variable modification parameters w […]
Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
[…] MS/MS data were collected using a MALDI-QIT-TOF MS (AXIMA Resonance and MALDI-7090TM; Shimadzu). Spectra were exported to the Mascot search engine (Matrix Science, Boston, MA) using the following search parameters: taxonomy = Homo sapiens; database = SwissProt; MS tolerance = 0.2 Da; MS/MS tolerance = 0.3 Da; enzyme = trypsi […]
Simulated microgravity induces a cellular regression of the mature phenotype in human primary osteoblasts
[…] Compass DataAnalysis 4.0 software (Bruker Daltonics) was used for data processing. Generated mgf files were then merged per lane and used for database search (SwissProt, version 20150612) through the MASCOT Daemon application included in an in-house MASCOT server (version 2.5, Matrix Science, London, UK) with the following constraints: taxonomy = Homo sapiens (20,207 sequences); enzyme = trypsin; […]
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