massWolf protocols

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massWolf statistics

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massWolf specifications


Unique identifier OMICS_19522
Name massWolf
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input format MassLynx
Output format mzXML
Operating system Windows
Programming languages C++
Computer skills Advanced
Version 4.3.1
Waters' MassLynx
Maintained No


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massWolf in pipelines

PMCID: 4221630
PMID: 25372836
DOI: 10.1371/journal.pone.0111016

[…] maldi-tof mass spectrometer (waters corp., milford, ma and ab sciex, farmingham, ma). twenty spectra were combined for analysis and were converted from masslynx.raw directories to.mzxml files using masswolf or from.mgf to.mzxml using proteowizard msconvert for import into an r computing environment, version 3.01, for analysis., once the data were imported into an r environment, the xcms – […]

PMCID: 2649809
PMID: 18984577
DOI: 10.1074/mcp.M800231-MCP200

[…] obtained by q-tof micro spectrometer from waters micromass. the ms duty cycle was set at 1,1,4 as described in detail in the supplemental text, part a. these data were converted to mzxml files using masswolf 1.02 with the waters datafile access component (dac) library. following the database search using the common criteria, quantitation was performed using a spectral count approach (), […]

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massWolf in publications

PMCID: 5762299
PMID: 29340031
DOI: 10.18632/oncotarget.22424

[…] residues were considered as variable modifications. peptides were identified with a q-value of 0.05. for silac quantification the raw data files were converted to *.mzxml in profile mode using masswolf file converter. further data processing was carried out with toppas. the *.mzxml files were converted to *.mzml. for subsequent data processing the mzml files were filtered (only ms1 level, […]

PMCID: 5678157
PMID: 29118436
DOI: 10.1038/s41598-017-15347-z

[…] in the current study, protein quantitation was performed by a label-free approach using the ideal-q software. the spectral raw data were first converted into mzxml format using a computational tool, masswolf v4.0 ( (masslynx converter)). the obtained mzxml data coupled with mascot search results in xml format were then submitted to ideal-q […]

PMCID: 4773761
PMID: 26932318
DOI: 10.1038/srep22526

[…] rats for each matrix., xcms online ( was used for univariate data analysis. raw data files were initially converted to mzdata files and the lockmass was removed using masswolf 4.3.1 and the chemhelper package in r version 3.2.0. function 1 ms data was uploaded to xcms online and the data was processed as a multi-group experiment using the default uplc with g2s ms […]

PMCID: 4619651
PMID: 26492191
DOI: 10.1371/journal.pone.0140823

[…] is about 3 μg thus it was about 1 μg of the peptides injected into the system for each of lc-ms analysis., the spectra generated from the lc-ms/ms were first converted into the mzxml format using masswolf (version 4.3.1). the ms/ms spectra in the mzxml format were subjected to spectral processing using the uniqua software, version 1.1 []. for ms/ms spectral processing, the parameters […]

PMCID: 4524895
PMID: 26300899
DOI: 10.3389/fpls.2015.00602

[…] ion mode for a period of 60 s each and intensity data was averaged and then analyzed according to garcia-flores et al. (unpublished data). the raw ms spectra files were converted to *.mzxml using masswolf v 1.4. analysis of mass spectra was performed with the openms/topp suite, version 1.8.0 and further processed with the free statistical r software (, microarray […]

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