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|Alternative name||Mutation Database for Parkinson's Disease|
|Restrictions to use||None|
- Homo sapiens
Publication for Mutation Database for Parkinson's Disease
A chemical genetic screen uncovers a small molecule enhancer of the N acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis
[…] le deionized water. For seedling growth assays, seeds were plated on solid 0.5 X MS salts nutrient media supplemented with 1% sucrose alone or with 30, 35 or 40 μM NAE 12:0 and 30 μM, 50 μM or 100 μM MDPD. Plates were wrapped with parafilm, stratified for 48 h in dark at 4 °C and then incubated vertically in a growth room with 16-h-light/8-h-dark cycle (6 0 μmol.m−2.s−1) at 20 to 22 °C. Images for […]
MCMC ODPR: Primer design optimization using Markov Chain Monte Carlo sampling
[…] by at least one primer. Whereas this approach has the constraint that all input sequences have the same length as the primers and may be inadequate in practice, the Multiple Degenerate Primer Design (MDPD) allows the input sequences to have different lengths of greater than lmin and attempts to identify primers of length at least lmin and degeneracy dmax, allowing each sequence to be covered by at […]
The biosynthetic genes for prenylated phenazines are located at two different chromosomal loci of Streptomyces cinnamonensis DSM 1042
[…] C CAC CTA CGA GAC CCC (A/C/G/T)(C/T)T (A/C/G/T)TG (A/C/G/T)TG GCC‐3′) and CodeHMG_rev (5′‐CGC ATC AGC TCG CCG (G/C)(G/T)(A/G) TT(A/C/G/T) GT(C/T) TG‐3′); for mevalonate diphosphate decarboxylase gene MDPD_for (5′‐GAC CCT GGA CGT CTT CCC (A/C/G/T)AC (A/C/G/T)AC (A/C/G/T)AC‐3′) and MDPD_rev (5′‐GCG TTC CGC TCG GC(A/G/T) AT(C/T) TC(A/C/G/T) CC‐3′). […]
Designing multiple degenerate primers via consecutive pairwise alignments
[…] zes and maximum degeneracy values (Figure ).Comparing the run time of PAMPS and PT-MIPS shows that PAMPS is astonishingly faster than PT-MIPS (both software were run on a 2.4 GHz Intel® CPU): solving MDPD problem for 100 input sequences of length 50 nucleotides is about 3380 times faster using PAMPS compared to PT-MIPS (Figure ). This allows PAMPS to be used to design highly degenerate primers for […]
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