MegaBLAST specifications


Unique identifier OMICS_01435
Name MegaBLAST
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes



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  • person_outline Alejandro Schaffer <>

Additional information

MegaBLAST article

MegaBLAST citations

PMCID: 5923482

[…] 99% identity). spades all reads assembly contained a 751 bp contig. this contig was disregarded due to low coverage (<1.5×) and because it had no significant sequence similarity when queried (megablast) against the ncbi nr database., the next step in the workflow is the determination of termini position and packaging strategy and involves the following checkpoints: 5.are genome ends […]

PMCID: 5878103

[…] using the megablast algorithm 10. only orthobunyavirus sequences were retrieved among the first 100 hits with the higher score and lower e-values. the closest nucleotide sequence returned from the megablast analysis belongs to an oropouche virus isolated in peru 2008 (strain tvp-19256/iqe-7894 - kp795086) sharing 98% similarity, with 545/558 identical sites., recently, the international […]

PMCID: 5880176

[…] observed between the automatic annotation and pan-genome comparison were corrected based on the results of the aforementioned work when feasible., the second method was based on blasting (blastn megablast, ncbi database) relevant staphylococcus reference sequences, retrieved from genbank and originating from reviewed protein sequences in uniprot, against the whole genome sequences […]

PMCID: 5795418

[…] with orfs encoding proteins of unknown function, accounting for approximately 70% of all genes., no related phages within the wider sequenced population were identified by searches conducted with dc-megablast. however, coregenes comparisons of phages identified by tblastx demonstrate that these two phages share a maximum of 20% of proteins with members of the pakpunavirus and the pseudomonas […]

PMCID: 5813145

[…] v.1.8 (, for initial taxonomic identification of cultures, single‐pass rpb2 sequences were aligned to an in‐house rpb2 sequence database (leblanc et al., 2017) using megablast and tblastx v.2.5.0+ (altschul et al., 1997; camacho et al., 2009). alignments to reference rpb2 sequences were evaluated based on bit‐score. sequences aligning to non‐fusarium references […]

MegaBLAST institution(s)
National Center for Biotechnology Information, National Institutes of Health, Department of Health and Human Services, Rockville Pike, Bethesda, MD, USA
MegaBLAST funding source(s)
Supported by Intramural Research Program of the National Institutes of Health, NLM.

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