An open-access metabolome database for tomato fruit. The database was developed with LC-MS data and primarily contains semipolar metabolites. MoTo DB also contains metabolite information from the literature and includes a variety of tomato fruit extracts from cultivated, wild, and transgenic tomato varieties. To search the database for a compound of interest, the mass is entered, either as a range or an accurate value, along with the ionization mode. The mass accuracy can also be set to accommodate data from a variety of detectors (low or high accuracy). The results pane displays the projected formula, a link to any PubChem identifiers, and CAS number as well as a link to the retention time, calculated accurate mass, MS/MS fragments, UV/visible spectral information, and alternative names for the compound (if any) and literature references.