MetaMorph protocols

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MetaMorph specifications

Information


Unique identifier OMICS_06501
Name MetaMorph
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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MetaMorph in pipelines

 (34)
2018
PMCID: 5834689
PMID: 29440406
DOI: 10.1073/pnas.1716266115

[…] confocal microscope or elyra zeiss microscope with sim (63× objective under oil) and lasers exciting at 405 nm (41.5 mw), 488 nm (21.2 mw), and 561 nm (20.2 mw). image analysis was conducted using metamorph imaging series 7 software (universal imaging), imaris 7 (bitplane), or volocity (perkinelmer). images are presented as either single planes or projections, as stated in legends. we used […]

2017
PMCID: 5438137
PMID: 28542402
DOI: 10.1371/journal.pone.0177921

[…] an image stack (*.stk file). the variable-sized image stack contained positional metadata that allowed subsequent reconstruction into an image montage. the image stack was processed using standard metamorph filters and segmented into cell nuclei and cytoplasmic features using custom routines created using cellprofiler (broad institute, cambridge, ma, usa). the segmented images […]

2017
PMCID: 5461764
PMID: 28587682
DOI: 10.1186/s40478-017-0444-6

[…] the density of round shape giant trophoblasts, anonymized toluidin blue stained semithin sections were used. acquired tiff format images were opened in the computer-assisted image analysis station metamorph (roper scientific, evry, france). after calibration of the objective used for acquisitions, thickness and cell density were quantified using the integrated morphometric analysis tools. […]

2017
PMCID: 5473868
PMID: 28623274
DOI: 10.1038/s41598-017-03483-5

[…] with an uplansapo 100 ×/1.40 na objective (olympus) at 25 °c. digital images were obtained with an ixon charge-coupled device camera (andor technology, belfast, northern ireland) controlled by metamorph imaging software (molecular devices, sunnyvale, ca, usa)., to analyse the mobility of the laco spots, a z-series of nine planes at 2-μm intervals for 2- to 8-cell embryos and 1-μm intervals […]

2017
PMCID: 5474736
PMID: 28607494
DOI: 10.1038/ncomms15835

[…] using icy software (plugin spot detector) by delineating melanosomes with circles (15 pixels diameter)—which overlap the tyrp1 labelling at melanosomes. linescan analysis was performed using metamorph. pearson’s correlation coefficient between green and red channels intensities (gfp-rab6/mch-vamp7 and npy-venus/ rab6-aa2) was calculated using the jacop plugin of imagej fiji. as controls, […]


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MetaMorph in publications

 (7489)
PMCID: 5958132
PMID: 29773802
DOI: 10.1038/s41467-018-04418-y

[…] 50 µm of lyve-1+ lymphatic vessels. type i collagen deposition was quantified as a ratio of positively stained dermis and subcutaneous tissues within a fixed threshold to total tissue area using metamorph offline software (molecular devices; sunnyvale, ca). subcutaneous fibroadipose thickness was analyzed as the width of tissues bounded by the reticular dermis to the deep fascia in four […]

PMCID: 5955896
PMID: 29769570
DOI: 10.1038/s41598-018-25950-3

[…] images of each cross section was performed under a nikon eclipse 800 epifluorescence microscope (nikon instruments, melville, ny) with cool snap camera (roper scientific, tucson, az, usa) and metamorph software (v7.1, universal imaging, downington, pa). images were edited with adobe photoshop cs5 (adobe systems, mountain view, ca). both total and tunel-positive spermatogonia were recorded […]

PMCID: 5955950
PMID: 29769580
DOI: 10.1038/s41598-018-25940-5

[…] seeded on glass coverslips, with or without pap-1 for 24 h, fixed and stained with alexa-fluor 488 phalloidin (invitrogen) for 20 min together with hoechst. fluorescent images were processed using metamorph 7.6.5.0 software (molecular device, usa) and form factor was calculated according the formula 4π area/perimeter2., no production by microglia cultures was assessed by measuring nitrite […]

PMCID: 5954220
PMID: 29764944
DOI: 10.1128/mBio.00526-18

[…] were captured using a nikon ti eclipse microscope with a yokogawa csu-xi spinning disc confocal, a clara interline charged-couple-device camera, 60× (1.4 numerical aperture) plan apo objective, and metamorph software. two z-stacks were captured at 10-s intervals for 15 min per field of view. individual movements were then tracked using imagej software with the manual tracking plug-in. a minimum […]

PMCID: 5946395
PMID: 29760782
DOI: 10.1186/s13039-018-0380-6

[…] antifade solution (vector laboratories). the slides were observed under a fluorescence microscope (olympus bx53) equipped with a cooled ccd camera (photometrics coolsnap myo), processed by metamorph, metavue imaging series version 7.8 and edited with adobe photoshop cs 6., total chromosome length and percentage of cells showing properly dispersed chromosomes per slide were measured […]


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MetaMorph institution(s)
Molecular Devices, LLC, CA, USA

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