Metastats

[…] ac) between the cow-calf production groups compared to the feeder production groups for the commercial herds was 0.256381.Beta diversity, at the species level, among populations was assessed with the MetaStats function in Mothur (pairwise P-values provided in Additional file : Table S2 and differences in species prevalence by animal age for the conservation herds is provided in Additional file : T […]

[…] ere generated using QIIME, and were visualised using the function heatmap.2 in the R gplots library []. Comparisons between paired samples were performed using the non-parametric statistical software Metastats which corrects for multiple comparisons is robust for sparse data []. […]

[…] r) hypothesis testing was performed to test whether the differential clustering of the PBS samples in the dendrograms was significant. Differences in the 30 most abundant OTUs were assessed using the metastats command in mothur and p-values for differences in individual OTUs were corrected for multiple comparisons using Bonferroni correction. Values of p < 0.05 were considered statistically signif […]

[…] Wilcoxon rank-sum test was used to examine the significance of alpha diversity indices. Metastats [], described in detail in our previous studies [,], was used to obtain the relative abundance differences of microbial communities between groups (groups = 2, samples per group ≥ 3). The ob […]

[…] tion in “Vegan”. Similarity percentage (SIMPER) procedure implemented in “Vegan” was used to quantify the contribution of individual OTUs to the overall Bray-Curtis dissimilarity between the groups. “MetaStats” [] was used to test for OTUs and orders with statistically significant differential abundances between the exposure groups. Only OTUs containing more than 0.1% of the total number of reads […]

[…] s in the cecal digesta was created by the R package gplots. Relative abundances of taxonomy and OTU were compared between the control and BG-treated group using a permutation test via online software Metastats. The qPCR data were subjected to simple t-test. Significance level was set at 0.05. […]