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MethylQuant specifications


Unique identifier OMICS_23681
Name MethylQuant
Software type Pipeline/Workflow
Interface Command line interface
Restrictions to use None
Operating system Windows
Programming languages Python
Computer skills Advanced
Stability Stable
Maintained Yes


  • Plants and Fungi
    • Saccharomyces cerevisiae




No version available


  • person_outline Gene Hart-Smith

Publication for MethylQuant

MethylQuant citations


Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling

Nat Commun
PMCID: 4396391
PMID: 25849564
DOI: 10.1038/ncomms7758

[…] V.G. performed all the experiments and conceived the isomethionine labelling strategy. V.G. and O.A. designed the experiments. V.G., D.T. and O.A. analysed the data. V.G. wrote MethylQuant. A.G. provided the antibodies and expertise with immuno-affinity purifications. B.T. provided expertise with sample preparation and data analysis. V.G. and O.A. wrote the manuscript. […]


Combination of methylated DNA precipitation and methylation sensitive restriction enzymes (COMPARE MS) for the rapid, sensitive and quantitative detection of DNA methylation

Nucleic Acids Res
PMCID: 1363782
PMID: 16473842
DOI: 10.1093/nar/gnj022

[…] argeting the bisulfite induced sequence changes to specifically amplify either methylated or unmethylated alleles. Quantitative variations of this technique, such as MethyLight (), HeavyMethyl () and MethylQuant (), employ methylation-specific oligonucleotides in conjunction with Taqman probes or SYBR Green based real-time PCR amplification to quantitate alleles with a specific pattern of methylat […]


MSRE PCR for analysis of gene specific DNA methylation

Nucleic Acids Res
PMCID: 1145194
PMID: 15944447
DOI: 10.1093/nar/gni092

[…] es left unmodified by bisulfite treatment; MethyLight () detects methylation in 1–5 CpG sites covered by primers and probe; QAMA () measures methylation in a 14 bp fragment covered by a TaqMan probe; MethylQuant () assays methylation of a single cytosine in bisulfite-modified DNA. It appears that bisulfite sequencing () is the only technique that provides the methylation profile of each and every […]


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MethylQuant institution(s)
NSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia; Centre for Immunology and Infection, University of York, Heslington, York, UK
MethylQuant funding source(s)
Supported by the Australian Research Council (ARC), the UNSW School of Biotechnology and Biomolecular Sciences, and an Australian Postgraduate Award.

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