MethylViewer specifications

Information


Unique identifier OMICS_00608
Name MethylViewer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Michael P. Kladde <>

Publication for MethylViewer

MethylViewer in publications

 (3)
PMCID: 5714208
PMID: 29608678
DOI: 10.1093/gbe/evx212

[…] the qiaquick pcr purification kit (qiagen) and then cloned into vector puc19/smai (thermo scientific) for sequencing. a minimum of ten different clones per species were sequenced and analysed by methylviewer software ()., genomic dna was digested with the restriction enzymes bamhi, hpaii, and psti and the resulting fragments were separated in a 1.2% agarose gel and blotted onto a biodyne b […]

PMCID: 3944461
PMID: 23818228
DOI: 10.1002/emmm.201302827

[…] pgem t-easy vector (promega, madison, wi) followed by transformation of top10 cells (invitrogen). individual clones were sequenced and data was analysed using sequencher for sequence alignment and methylviewer to assign site-specific methylation status (pardo et al, ). molecules with <97% conversion efficiency were excluded., statistical analyses were performed in graphpad prizm 5.0 […]

PMCID: 3597696
PMID: 23361465
DOI: 10.1093/nar/gkt033

[…] the genomic sequences corresponding to bgs amplicons are listed in supplementary table s2. sequence files in abi format were aligned in sequencher™ (genecodes) and are available on request. methylviewer [http://dna.leeds.ac.uk/methylviewer; ()] was used to ascertain percent cytosine conversion and tabulate methylation status of each site in each sequence read (i.e. molecule). molecules […]


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MethylViewer institution(s)
Department of Biochemistry and Molecular Biology, University of Florida Shands Cancer Center Program in Cancer Genetics, Epigenetics and Tumor Virology, Gainesville, USA

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