MFEprimer statistics

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Associated diseases

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MFEprimer specifications


Unique identifier OMICS_02355
Name MFEprimer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS
Programming languages Python, Shell (Bash)
Computer skills Advanced
Version 2.0
Stability Stable
Maintained Yes


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  • person_outline Chenggang Zhang <>

Publications for MFEprimer

MFEprimer in publications

PMCID: 5792648
PMID: 29386622
DOI: 10.1038/s41598-018-20414-0

[…] from rnammer (v1.2) software. sequences including the v4 region were recovered by considering the start/end positions of the alignment on hidden markov models. a “virtual pcr” was performed using mfeprimer-2.0 in order to verify the ability of universal primers to match each sequence. mfeprimer-2.0 software was launched on each 16s rrna sequence using an automated pipeline which also parsed […]

PMCID: 5471038
PMID: 28402272
DOI: 10.18632/oncotarget.16626

[…] conformations in each cluster were attained from the docking log files (dlg)., primers for upa, pai-1, cxcr4 and mmp-9 were designed using primer3 ( the mfeprimer-2.0 online tool ( was used to test primer specificity and usability. amplification specificities were rechecked with primer blast […]

PMCID: 5153897
PMID: 27955616
DOI: 10.1186/s12864-016-3375-x

[…] of the length of lcs and lss revealed that 39 and 72% of locations, respectively, in the mitochondrial genome are more specific than the locations chosen for these primers. in-silico pcr, using mfeprimer-3.0 [] against the human genome, shows that these primers can produce 24 amplicons from the human nuclear genome (see additional file for more information).table 2 fig. 3 , the best […]

PMCID: 5067579
PMID: 27752099
DOI: 10.1038/srep35520

[…] at 95 °c for 5 s, 60 °c for 1 min, cooling at 50 °c for 30 s. at the end of the qpcr runs, a melting curve was created. the primers were designed and verified using the primer 3, oligo 7.0, and mfeprimer-2.0. the primers used in this study are shown in , and each sample was tested in triplicate. the mrna level was normalised to glyceraldehyde 3-phosphate dehydrogenase (gapdh), […]

PMCID: 5133970
PMID: 27711076
DOI: 10.1038/cddis.2016.299

[…] primers for each gene under study were designed using primer-blast ( and their specificity checked using mfe, we studied stem cells from human exfoliated deciduous teeth (shed) obtained from three tcs subjects and from three control individuals subjected to reconstructive plastic surgery […]

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MFEprimer institution(s)
Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing, China; College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu, China; Beijing Institute of Health Service and Medical Information, Beijing, China
MFEprimer funding source(s)
Supported by the National Basic Research Project (973 program) [2012CB518200]; General Program [30900862, 30973107, 81070741, 81172770] of the Natural Science Foundation of China; State Key Laboratory of Proteomics of China [SKLP-O201104, SKLP-K201004, SKLP-O201002]; and Special Key Programs for Science and Technology of China [2012ZX09102301-016].

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