MFEprimer statistics

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MFEprimer specifications

Information


Unique identifier OMICS_02355
Name MFEprimer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS
Programming languages Python, Shell (Bash)
Computer skills Advanced
Version 2.0
Stability Stable
Maintained Yes

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Documentation


Maintainer


  • person_outline Chenggang Zhang <>

Publications for MFEprimer

MFEprimer in publications

 (18)
PMCID: 5792648
PMID: 29386622
DOI: 10.1038/s41598-018-20414-0

[…] from rnammer (v1.2) software. sequences including the v4 region were recovered by considering the start/end positions of the alignment on hidden markov models. a “virtual pcr” was performed using mfeprimer-2.0 in order to verify the ability of universal primers to match each sequence. mfeprimer-2.0 software was launched on each 16s rrna sequence using an automated pipeline which also parsed […]

PMCID: 5471038
PMID: 28402272
DOI: 10.18632/oncotarget.16626

[…] conformations in each cluster were attained from the docking log files (dlg)., primers for upa, pai-1, cxcr4 and mmp-9 were designed using primer3 (http://bioinfo.ut.ee/primer3-0.4.0/primer3/). the mfeprimer-2.0 online tool (http://biocompute.bmi.ac.cn/czlab/mfeprimer-2.0/) was used to test primer specificity and usability. amplification specificities were rechecked with primer blast […]

PMCID: 5153897
PMID: 27955616
DOI: 10.1186/s12864-016-3375-x

[…] of the length of lcs and lss revealed that 39 and 72% of locations, respectively, in the mitochondrial genome are more specific than the locations chosen for these primers. in-silico pcr, using mfeprimer-3.0 [] against the human genome, shows that these primers can produce 24 amplicons from the human nuclear genome (see additional file for more information).table 2 fig. 3 , the best […]

PMCID: 5067579
PMID: 27752099
DOI: 10.1038/srep35520

[…] at 95 °c for 5 s, 60 °c for 1 min, cooling at 50 °c for 30 s. at the end of the qpcr runs, a melting curve was created. the primers were designed and verified using the primer 3, oligo 7.0, and mfeprimer-2.0. the primers used in this study are shown in , and each sample was tested in triplicate. the mrna level was normalised to glyceraldehyde 3-phosphate dehydrogenase (gapdh), […]

PMCID: 5133970
PMID: 27711076
DOI: 10.1038/cddis.2016.299

[…] primers for each gene under study were designed using primer-blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and their specificity checked using mfe http://biocompute.bmi.ac.cn/czlab/mfeprimer-2.0/., we studied stem cells from human exfoliated deciduous teeth (shed) obtained from three tcs subjects and from three control individuals subjected to reconstructive plastic surgery […]


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MFEprimer institution(s)
Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing, China; College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu, China; Beijing Institute of Health Service and Medical Information, Beijing, China
MFEprimer funding source(s)
Supported by the National Basic Research Project (973 program) [2012CB518200]; General Program [30900862, 30973107, 81070741, 81172770] of the Natural Science Foundation of China; State Key Laboratory of Proteomics of China [SKLP-O201104, SKLP-K201004, SKLP-O201002]; and Special Key Programs for Science and Technology of China [2012ZX09102301-016].

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