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MGC specifications

Information


Unique identifier OMICS_15165
Name MGC
Alternative name Mammalian Gene Collection
Restrictions to use None
Community driven No
Data access Browse
User data submission Not allowed
Maintained Yes

Maintainer


  • person_outline Mammalian Gene Collection Team

Publications for Mammalian Gene Collection

MGC citations

 (25)
library_books

Establishment of a Wheat Cell Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins

2016
PLoS One
PMCID: 4889105
PMID: 27249653
DOI: 10.1371/journal.pone.0156718

[…] ed the assay to detect direct interaction between E3 and target protein so as to identify E3 for a target protein. At the beginning of this study, 232 human cDNAs encoding human E3 were selected from mammalian gene collection (MGC) clones covering more than 20,000 human full-length cDNAs without redundancy. In addition, cDNAs for three human and 23 mouse E3s not contained in the MGC clones were ob […]

library_books

Novel Autoantigens Associated with Lupus Nephritis

2015
PLoS One
PMCID: 4476694
PMID: 26098692
DOI: 10.1371/journal.pone.0126564

[…] ins in the extracellular space” in the Gene Ontology database, as tentative autoantigens. A total of 2,296 genes encoding these target proteins were translated from a human full-length cDNA resource (mammalian gene collection, MGC clone, DANAFORM, Tokyo, Japan). From the plasmid-inserted cDNA, transcription templates were synthesized by split-primer polymerase chain reaction (PCR). The promoter an […]

library_books

The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

2014
PLoS One
PMCID: 4227841
PMID: 25386923
DOI: 10.1371/journal.pone.0112643

[…] . Although present in many expression vectors, transcription terminators can be included downstream of the transcribed gene if instability is predicted . Finally, sources of cDNA can be found in the Mammalian Gene Collection (http://mgc.nci.nih.gov/) and at the home page of Culture Collection of the World (http://www.ecotao.com/holism/agric/hpcc.html).No expression system is generic for all targe […]

library_books

The Maize TFome – development of a transcription factor open reading frame collection for functional genomics

2014
Plant J
PMCID: 4283594
PMID: 25053252
DOI: 10.1111/tpj.12623

[…] Seq expression data available at qTeller (http://qteller.com/qteller3/). This approach, which is also referred to as directed RT-PCR or ‘rescue PCR’, was previously used to generate human ORFs in the Mammalian Gene Collection project (Temple et al., ). While a variety of maize tissues were used to ensure gene amplification, young seedlings proved to be a reliable source of transcripts for about 37 […]

call_split

Proteomic analysis of fibroblastema formation in regenerating hind limbs of Xenopus laevis froglets and comparison to axolotl

2014
BMC Dev Biol
PMCID: 4222900
PMID: 25063185
DOI: 10.1186/1471-213X-14-32
call_split See protocol

[…] Proteins not recognized by the algorithm were manually curated. NCBI blastp [] was used to match the sequences of hypothetical/novel/unknown/unnamed/Locus (LOC)/NIH Mammalian Gene Collection (MGC) proteins against the 'vertebrata' category in blast (taxid: 7742) to identify their closest neighbors. Only the proteins having 90% peptide ID confidence and with FDR < […]

library_books

ASPic GeneID: A Lightweight Pipeline for Gene Prediction and Alternative Isoforms Detection

2013
Biomed Res Int
PMCID: 3838850
PMID: 24308000
DOI: 10.1155/2013/502827

[…] to align mRNA sequences to genome [, ]. As shown in , ASPic outperforms PAIRAGON-any in predicting correct introns. Considering that PAIRAGON-any aligns only high quality sequences from the full ORF Mammalian Gene Collection (MGC) [] and from the human RefSeq database, ASPic's performance which is based only on ESTs is even more remarkable.ASPic is not highly specific at the transcript level wher […]

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MGC institution(s)
National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA

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