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MicroGen specifications


Unique identifier OMICS_00867
Name MicroGen
Restrictions to use None
Maintained No


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MicroGen citations


Metagenomic Analysis of Therapeutic PYO Phage Cocktails from 1997 to 2014

PMCID: 5707535
PMID: 29099783
DOI: 10.3390/v9110328

[…] %. This relates to the continued scarceness of phage genome representation in public databases compared to bacterial sequences []. A previous study from 2013 was able to map 61% of the reads from the Microgen ColiProteus cocktail to public genomes [].For PYO97, 17% of the bins were not predicted to be of phage origin, while for PYO2014 the corresponding percentage was 10. When predicting if bins w […]


Complete Genome Sequence of Mycobacterium bovis Strain BCG 1 (Russia)

Genome Announc
PMCID: 4816620
PMID: 27034492
DOI: 10.1128/genomeA.00182-16
call_split See protocol

[…] whole-genome sequencing and comparison of all BCG Russia strains are extremely important. Here, we report the complete genome sequence of M. bovis BCG-1, isolated from a working seed lot used by NPO Microgen (Russia) for vaccine production.Genomic DNA was isolated according to van Embden et al (). A whole-genome shotgun library, prepared with a GS FLX Titanium rapid library preparation it (Roche […]


Roles of thermophilic thiosulfate reducing bacteria and methanogenic archaea in the biocorrosion of oil pipelines

Front Microbiol
PMCID: 3944610
PMID: 24639674
DOI: 10.3389/fmicb.2014.00089
call_split See protocol

[…] in, and grown overnight at 37°C. Plasmid DNA was purified from the transformed cells and sequenced using the M13F and M13R vector flanking regions as priming sites on an ABI 3730 capillary sequencer (Microgen: Laboratory for Genomics and Bioinformatics, Oklahoma City, OK).The consensus bacterial 16S rRNA gene sequences were obtained using Sequencher version 5.1 (Gene Codes, Ann Arbor, MI). The sof […]


Adenoviruses Associated with Acute Diarrhea in Children in Beijing, China

PLoS One
PMCID: 3923065
PMID: 24533149
DOI: 10.1371/journal.pone.0088791
call_split See protocol

[…] ucts were verified by sequencing and then analyzed at the NCBI website (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi). The specimens were also tested for rotavirus using the Rota/Adenoscreen Dipstick (Microgen Bioproducts Ltd., Camberley, Surrey, UK). The sequences of Ad 41 that were amplified from fecal specimens were aligned with CLUSTAL W software. The neighbor-joining (NJ) algorithm in MEGA4 wa […]


Exposure of Soil Microbial Communities to Chromium and Arsenic Alters Their Diversity and Structure

PLoS One
PMCID: 3386950
PMID: 22768219
DOI: 10.1371/journal.pone.0040059
call_split See protocol

[…] lones were randomly selected and checked for inserts by PCR prior to sequencing. Clone libraries, consisting of 48 clones for each gene and site, were replicated from stocks before being sequenced by Microgen (Microgen, Oklahoma City, OK). Sequencing was performed using an ABI 3730xl capillary sequencer (Applied Biosystems) Sequences underwent quality screening, primer removal, and binning into Sh […]


Diversity, mobility, and structural and functional evolution of group II introns carrying an unusual 3' extension

BMC Res Notes
PMCID: 3261151
PMID: 22204608
DOI: 10.1186/1756-0500-4-564

[…] having a similar extension. BLASTN was run with default parameters, except that the nucleotide match reward was set to 2 (-r 2). The genome sequence of B. thuringiensis kurstaki HD1 available at the Microgen website (Laboratory for Genomics and Bioinformatics, University of Oklahoma Health Sciences Center, Oklahoma City, USA; http://www.micro-gen.ouhsc.edu/b_thuring/b_thuringiensis_home.htm) was […]


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MicroGen institution(s)
BioMedical Informatics Laboratory, Bioengineering Department, Politecnico di Milano, Milan, Italy

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