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Protocols

MicroVigene specifications

Information


Unique identifier OMICS_00845
Name MicroVigene
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
License Commercial
Computer skills Medium
Version 3.0
Stability Stable
Free trial Yes
Maintained Yes

Versioning


No version available

Documentation


Maintainer


  • person_outline MicroVigene Team

MicroVigene citations

 (73)
library_books

Preclinical evaluation and reverse phase protein Array based profiling of PI3K and MEK inhibitors in endometrial carcinoma in vitro

2018
BMC Cancer
PMCID: 5807759
PMID: 29426295
DOI: 10.1186/s12885-018-4035-0

[…] mplified using a Dakocytomation-catalysed system (Dako) and visualized by chromogenic detection (using diaminobenzidine). Slides were then scanned, analysed, and quantified using customized software (Microvigene, VigeneTech Inc.) to generate spot intensities.Heat-maps were generated from normalized data using unsupervised hierarchical clustering analysis performed with publicly available Morpheus […]

library_books

Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection

2018
PLoS One
PMCID: 5800665
PMID: 29408900
DOI: 10.1371/journal.pone.0191983

[…] s. Antibody and Sypro Ruby stained slides were scanned on a Tecan laser scanner (TECAN, Mönnedorf, Switzerland) using the 620nm and 580nm weight length channel, respectively.Images were analyzed with MicroVigene Software Version 5.1.0.0 (Vigenetech). The software performs spot finding along with subtraction of the local background and non-specific binding generated by the secondary antibody. Each […]

library_books

Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non small cell lung cancer

2018
Oncotarget
PMCID: 5865668
PMID: 29581842
DOI: 10.18632/oncotarget.24361

[…] We used MicroVigene software (VigeneTech, Carlisle, MA) and an in-house R-package [] to measure spot intensity. Quantification was performed utilizing a SuperCurve method which uses MicroVigene software (Vige […]

call_split

Cathepsin G Is Expressed by Acute Lymphoblastic Leukemia and Is a Potential Immunotherapeutic Target

2018
Front Immunol
PMCID: 5790053
PMID: 29422892
DOI: 10.3389/fimmu.2017.01975
call_split See protocol

[…] zation and expression controls. Slides were stained with 232 strictly validated primary antibodies, including one against CG (Abcam ab8816, Cambridge, MA, USA). The stained slides were analyzed using MicroVigene software (Vigene Tech, Carlisle, MA, USA) to produce quantified data. […]

library_books

Nonlinear mixed effects dose response modeling in high throughput drug screens: application to melanoma cell line analysis

2017
Oncotarget
PMCID: 5797032
PMID: 29435161
DOI: 10.18632/oncotarget.23495

[…] e amount of protein present in each sample. Antibody stained slides were scanned using the Tecan laser scanner (Tecan PowerScanner Tecan group Ltd., Männedorf, Switzerland). Images were analyzed with MicroVigene 5.1.0.0 (Vigenetech, Carlisle, MA) as previously described []. Because the output of the RPPA platform is quantitative, the intensity values obtained from the analysis were reported on a c […]

library_books

Protein activation mapping of human sun protected epidermis after an acute dose of erythemic solar simulated light

2017
PMCID: 5695572
PMID: 29167824
DOI: 10.1038/s41698-017-0037-7

[…] d individually on the NovaRay scanner (Alpha Innotech) or the Vidar Scanner (Vidar Systems, Herndon, VA). The TIF images for antibody-stained slides and Sypro-stained slide images were analyzed using MicroVigene v2.9.9.9 (VigeneTech, Carlisle, MA, USA). Briefly, Microvigene performed spot finding, local background subtraction, replicate averaging and total protein normalization, producing a single […]

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MicroVigene institution(s)
VigeneTech Inc., Carlisle, MA, USA

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