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chevron_left Differential expression detection Gene expression clustering Background correction Bioinformatics workflows chevron_right
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MIDAW specifications

Information


Unique identifier OMICS_14500
Name MIDAW
Interface Web user interface
Restrictions to use None
Input data A gene structure.
Input format TXT
Programming languages Perl, PHP
License GNU General Public License version 3.0
Computer skills Basic
Stability No
Maintained No

Maintainer


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Publication for MIDAW

MIDAW citations

 (8)
library_books

Involvement of MicroRNAs in the Regulation of Muscle Wasting during Catabolic Conditions*

2014
PMCID: 4139209
PMID: 24891504
DOI: 10.1074/jbc.M114.561845

[…] number gse52676., interarray normalization of expression levels performed with loessm+gpa for mirna experiments and a within-array-normalization, using the lowess method, was performed by using the midaw tool for gene expression profiling (). normalization function was applied to mirna expression data of all experiments and then values of spot replicates within arrays were averaged., cluster […]

library_books

MicroRNA Dependent Regulation of Transcription in Non Small Cell Lung Cancer

2014
PMCID: 3953115
PMID: 24625834
DOI: 10.1371/journal.pone.0090524

[…] were performed using the reciprocal fluorochrome labeling., to detect differentially expressed genes between the two histological subtypes, two types of analysis were undertaken with the midaw tool . first, a t-test was performed with false discovery rate (fdr) control estimated using the single-step bonferroni procedure. genes that passed the t-test filter were subjected to a second […]

library_books

Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae

2013
PMCID: 3876060
PMID: 24351863
DOI: 10.3390/ijms141223516

[…] were used for microarray analysis. the six microarray data were normalized using the quantile method [] with webarraydb online microarray data analysis []. data were filtered and assessed by the midaw online analysis program [] using the method of weighted k-nearest neighbor []. t-tests for microarray data were performed by a multiexperiment viewer (mev) software package (version 4.5, […]

library_books

Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae

2013
PMCID: 3676858
PMID: 23698783
DOI: 10.3390/ijms140510626

[…] microarray analysis. the six microarray data were normalized using the quantile normalization method [] with webarraydb (http://www.webarraydb.org/webarray/) [] and were filtered and assessed by the midaw online analysis program (http://www.webarraydb.org/webarray/) [] using the method of weighted k-nearest neighbor []. t-tests and hierarchical cluster analyses of the significantly […]

library_books

New miRNA labeling method for bead based quantification

2010
PMCID: 2900262
PMID: 20553585
DOI: 10.1186/1471-2199-11-44

[…] the mirvana labeling kit (ambion) and amine-reactive dyes (ge healthcare) as recommended by the manufacturer's protocol []. normalization of expression levels of all spot replicates was performed by midaw []. principal component analysis, cluster analysis and profile similarity searching were performed with tmev software []. one and two class significance analysis of microarray (sam) allowed […]

library_books

Filling gaps in PPAR alpha signaling through comparative nutrigenomics analysis

2009
PMCID: 2801700
PMID: 20003344
DOI: 10.1186/1471-2164-10-596

[…] affymetrix probe sets in order to have an efficient and up-to-date genome annotation of array features. raw data derived by oligo microarray were normalized using lowess algorithm exploiting midaw web tool []. in order to identify differentially expressed gene (hereafter degs) we performed sam test [], a moderated t-test with permutational approach. p-values and then q-values (false […]


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MIDAW institution(s)
Dipartimento di Biologia, CRIBI Biotechnology Centre, Universita degli Studi di Padova, Padua, Italy
MIDAW funding source(s)
This work was supported by the Fondazione Telethon ONLUS; the Ministero del l'Università e della Ricerca Scientifica (grants FIRB and COFIN) and by the Fondazione della Cassa di Risparmio di Verona–Vicenza–Belluno–Ancona.

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