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Protocols

MiNA specifications

Information


Unique identifier OMICS_19398
Name MiNA
Alternative name Mitochondrial Network Analysis
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Requirements
FIJI or ImageJ
Maintained Yes

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Versioning


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Maintainers


  • person_outline Jeffrey Stuart
  • person_outline Andrew Valente

Publication for Mitochondrial Network Analysis

MiNA citations

 (4)
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Slower Dynamics and Aged Mitochondria in Sporadic Alzheimer's Disease

2017
PMCID: 5672147
PMID: 29201274
DOI: 10.1155/2017/9302761
call_split See protocol

[…] reatments cells were fixed with 4% paraformaldehyde (PFA) and the mitochondrial pattern was observed with an Axioskop2 plus microscope coupled to a CCD color camera (Zeiss). For analysis, we used the Mitochondrial Network Analysis (MiNa) toolset, a combination of different ImageJ macros that allows the semiautomated analysis of mitochondrial networks in cultured mammalian cells []. Briefly, the im […]

call_split

Functionally and morphologically damaged mitochondria observed in auditory cells under senescence inducing stress

2017
PMCID: 5445612
PMID: 28649420
DOI: 10.1038/s41514-017-0002-2
call_split See protocol

[…] h parts of the cytoplasm. The red images are band-pass filtered image for mitochondrial shape, and the green lines are the filtered and skeletonized image for mitochondrial network (Fig. ). Regarding mitochondrial network analysis, the objects were skeletonized using standard processing operations (medial axis transform), which involved an intensity threshold, followed by thinning and then pruning […]

library_books

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance

2014
PMCID: 3999722
PMID: 24727571
DOI: 10.1093/brain/awu060

[…] × 512 with perfect voxel settings (x, y, z, 0.12 μm). Sixty-nine z-planes across 8 μm were captured to allow for a 3D reconstruction of mitochondrial networks from individual cells. Deconvolution and mitochondrial network analysis was performed using Huygens Essentials software (SVI). Fifty cells were imaged per individual cell line. […]

library_books

Mitochondrial Dynamics Associated with Oxygen Glucose Deprivation in Rat Primary Neuronal Cultures

2013
PLoS One
PMCID: 3642144
PMID: 23658809
DOI: 10.1371/journal.pone.0063206

[…] rk with live mitochondrial staining in neurons we investigated 50–100 cells/experiment; at least three independent experiments were averaged using untreated normoxic controls for each series. For the mitochondrial network analysis we defined four cell types: (1) normal, tubular/short, tubular; (2) rounded; (3) highly interconnected/long, tubular; and (4) poorly labeled. Please note that our cultur […]

Citations

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MiNA institution(s)
Department of Biology, Brock University, St. Catharines, ON, Canada; Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON, Canada
MiNA funding source(s)
Supported by a Natural Science and Engineering Research Council (NSERC) Discovery Grant (RGPIN-2015- 05645).

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