An online tool that can simultaneously search up to 100 sequences for novel microRNAs (miRNAs) in multiple organisms. miREval 2.0 uses multiple published in silico approaches to detect miRNAs in sequences of interest. This tool can be used to discover miRNAs from DNA sequences or to validate candidates from sequencing data. miREval accepts up to 100 sequences of DNA or RNA in FASTA format. Comprehensive analysis is available for 31 species; partial analysis, including secondary structure prediction and alignment to known miRNAs, is still available for other species.