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An online tool that can simultaneously search up to 100 sequences for novel microRNAs (miRNAs) in multiple organisms. miREval 2.0 uses multiple published in silico approaches to detect miRNAs in sequences of interest. This tool can be used to discover miRNAs from DNA sequences or to validate candidates from sequencing data. miREval accepts up to 100 sequences of DNA or RNA in FASTA format. Comprehensive analysis is available for 31 species; partial analysis, including secondary structure prediction and alignment to known miRNAs, is still available for other species.

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miREval classification

miREval specifications

Web user interface
Computer skills:
Restrictions to use:

miREval distribution

miREval support


  • William Ritchie <>


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Bioinformatics Laboratory, Centenary Institute, University of Sydney, Sydney, New South Wales, Australia; Gene and Stem Cell Therapy Program, Centenary Institute, University of Sydney, Sydney, New South Wales, Australia; Cell and Molecular Therapies, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia

Funding source(s)

Australian National Health and Medical Research Council [Training Fellowship 571156], Cancer Institute NSW [career Development Fellowship 12/CDF/2-28], Cancer Council NSW [Project Grant 1006260], the Rebecca L. Cooper Foundation and the Cell and Gene Trust [Cure The Future]

Link to literature

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