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miRprimer | A tool for design of primers for microRNA-specific quantitative RT-qPCR

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Designs primers for polymerase chain reaction (PCR) amplification of microRNAs. miRprimer generates a number of putative primers based on an interpretation of the guidelines for manual primer design into computer language. The availability of automated primer design makes this method an even more attractive option for quantification of microRNA expression. Primers designed with this algorithm were tested in different experiments and have the same success rate as manually designed primers.

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miRprimer classification

miRprimer specifications

Unique identifier:
OMICS_02311
Interface:
Command line interface
Input data:
A list of microRNA names and sequences.
Operating system:
Windows
License:
Apache License version 2.0, GNU General Public License version 2.0
Version:
2.0
Maintained:
Yes
Software type:
Package/Module
Restrictions to use:
Academic or non-commercial use
Input format:
FASTA
Programming languages:
Ruby
Computer skills:
Advanced
Stability:
Stable

miRprimer distribution

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miRprimer support

Maintainer

  • Peter Busk <>

Credits

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Publications

Institution(s)

Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University Copenhagen, Copenhagen, Denmark

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