MiToBo specifications


Unique identifier OMICS_13124
Name MiToBo
Alternative name Microscope Image Analysis Toolbox
Software type Package/Module, Toolkit/Suite
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Maintained Yes


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  • person_outline Birgit Möller <>

Additional information

Publications for Microscope Image Analysis Toolbox

MiToBo in publications

PMCID: 5354766
PMID: 28038455
DOI: 10.18632/oncotarget.14206

[…] chemical, ann arbor, mi) and photographed initially (t = 0 h), and then up to 48 h with a 4x objective. image analysis was performed using imagej software [] with the scratch assay analyzer from the mitobo plug in. three independent experiments were performed on each cell line., cell proliferation was quantified using the cyquant® assay (invitrogen™) according to the manufacturer's instructions. […]

PMCID: 5106514
PMID: 27720906
DOI: 10.1016/j.stemcr.2016.09.006

[…] the colony, after being washed twice with medium, and marked. the scratch was imaged 18 hr later in the marked location. the scratch area was calculated using the scratch assay analyzer plug-in from mitobo toolbox for imagej ()., after 2 days of mesendodermal differentiation, cells were crosslinked with 0.8% formaldehyde (sigma-aldrich) in pbs for 10 min. the cells were washed twice and lysed […]

PMCID: 5342565
PMID: 27634876
DOI: 10.18632/oncotarget.11992

[…] from a spot rt3 2mp monochrome camera (diagnostic instruments, inc.) attached to an eclipse ts100 (nikon) microscope using a 10x/0.30 plan fluor objective (nikon). scratch area was measured with the mitobo plug-in for imagej (fiji) using default settings., candidate mirnas targeting runx1 and runx2 were identified using targetscan v6.2 ( ipa ( qiagen) […]

PMCID: 5215748
PMID: 27375028
DOI: 10.1038/onc.2016.234

[…] added to the wounded cultures to inhibit cell proliferation. microscopy images were taken at the designated timepoints and subjected to color segmentation using the scratch assay analyzer plugin (mitobo suite) for imagej (nih, bethesda, md, usa). the color segmentation procedure produced black pixels for area covered by cells, and pixel coverage was used to generate the graphs., proliferation […]

PMCID: 4856984
PMID: 26917013
DOI: 10.1093/nar/gkw099

[…] of 3d cultures for each position and time point z-stacks were acquired and maximal projections were analyzed. automated cell tracking was performed using the ‘cellmigrationanalyzer’ tool of the mitobo ( package for imagej ( to determine the mean speed [μm/min] of those single cells observed over a time period […]

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MiToBo institution(s)
Institute of Computer Science, Martin Luther University Halle-Wittenberg, Halle, Germany; Institute of Molecular Medicine, Martin Luther University Halle-Wittenberg, Halle, Germany

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