MiToBo statistics

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Citations per year

Number of citations per year for the bioinformatics software tool MiToBo

Tool usage distribution map

This map represents all the scientific publications referring to MiToBo per scientific context
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Associated diseases

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Popular tool citations

chevron_left Image-analysis libraries Morphometric analysis Scratch assay Image visualization chevron_right
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MiToBo specifications


Unique identifier OMICS_13124
Name MiToBo
Alternative name Microscope Image Analysis Toolbox
Software type Package/Module, Toolkit/Suite
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Maintained Yes


  • PaCeQuant




No version available



  • person_outline Birgit Möller

Additional information

Publications for Microscope Image Analysis Toolbox

MiToBo citations


Morphometrics of complex cell shapes: lobe contribution elliptic Fourier analysis (LOCO EFA)

PMCID: 5897594
PMID: 29444894
DOI: 10.1242/dev.156778

[…] nts present PCs that are more elongated or have shallower lobes, but which occur at a similar spatial frequency (). Recognising the need for automatic and non-biased quantification of PCs, developed PaCeQuant, a software to define lobes and necks in a systematic way based on local curvature. Similarly to LobeFinder, it is highly sensitive to small variations in the shape contour, with the samplin […]


The expression of Nectin 4 on the surface of ovarian cancer cells alters their ability to adhere, migrate, aggregate, and proliferate

PMCID: 5354766
PMID: 28038455
DOI: 10.18632/oncotarget.14206
call_split See protocol

[…] Chemical, Ann Arbor, MI) and photographed initially (t = 0 h), and then up to 48 h with a 4X objective. Image analysis was performed using ImageJ software [] with the Scratch Assay Analyzer from the MiToBo plug in. Three independent experiments were performed on each cell line. […]


Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling

Stem Cell Reports
PMCID: 5106514
PMID: 27720906
DOI: 10.1016/j.stemcr.2016.09.006

[…] the colony, after being washed twice with medium, and marked. The scratch was imaged 18 hr later in the marked location. The scratch area was calculated using the Scratch Assay Analyzer plug-in from MiToBo toolbox for ImageJ (). […]


A microRNA/Runx1/Runx2 network regulates prostate tumor progression from onset to adenocarcinoma in TRAMP mice

PMCID: 5342565
PMID: 27634876
DOI: 10.18632/oncotarget.11992

[…] from a SPOT RT3 2Mp Monochrome camera (Diagnostic Instruments, Inc.) attached to an Eclipse TS100 (Nikon) microscope using a 10X/0.30 Plan Fluor objective (Nikon). Scratch area was measured with the MiToBo plug-in for ImageJ (Fiji) using default settings. […]


Keratin associated protein 5 5 controls cytoskeletal function and cancer cell vascular invasion

PMCID: 5215748
PMID: 27375028
DOI: 10.1038/onc.2016.234
call_split See protocol

[…] was added to the wounded cultures to inhibit cell proliferation. Microscopy images were taken at the designated timepoints and subjected to color segmentation using the Scratch Assay Analyzer plugin (MiToBo Suite) for ImageJ (NIH, Bethesda, MD, USA). The color segmentation procedure produced black pixels for area covered by cells, and pixel coverage was used to generate the graphs.Proliferation as […]


The oncogenic triangle of HMGA2, LIN28B and IGF2BP1 antagonizes tumor suppressive actions of the let 7 family

Nucleic Acids Res
PMCID: 4856984
PMID: 26917013
DOI: 10.1093/nar/gkw099

[…] lyses of 3D cultures for each position and time point z-stacks were acquired and maximal projections were analyzed. Automated cell tracking was performed using the ‘CellMigrationAnalyzer’ tool of the MiToBo ( package for ImageJ ( to determine the mean speed [μm/min] of those single cells observed over a time period of at […]

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MiToBo institution(s)
Institute of Computer Science, Martin Luther University Halle-Wittenberg, Halle, Germany; Institute of Molecular Medicine, Martin Luther University Halle-Wittenberg, Halle, Germany

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