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Quantifies biological hallmarks and includes mitochondrial morphology and nuclear condensation. IDOTMETER allows to measure a variety of cellular changes, mainly involving autophagy and cell survival determinations, with high throughput in a simple and easy manner. The main advantage of this tool is to automatically extract and analyze all of the information from images of interest in a batch mode. Using software for high-throughput cell image analysis offers researchers the possibility of performing comprehensive and precise analysis of a high number of images in an automated manner, making this routine task easier.