MITOPROT protocols

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MITOPROT specifications

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Unique identifier OMICS_07283
Name MITOPROT
Interface Web user interface
Restrictions to use None
Programming languages Pascal
Computer skills Basic
Stability Stable
Maintained No

Information


Unique identifier OMICS_07283
Name MITOPROT
Interface Command line interface
Restrictions to use None
Operating system Mac OS
Programming languages Pascal
Computer skills Advanced
Version 1.101
Stability Stable
Maintained No

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Publication for MITOPROT

MITOPROT in pipelines

 (4)
2017
PMCID: 5292771
PMID: 28179991
DOI: 10.1038/cddiscovery.2016.92

[…] sorting signals (http://ipsort.hgc.jp/). mitoprotii calculates the n-terminal protein region that can support a mitochondrial targeting sequence and the cleavage site (https://ihg.gsf.de/ihg/mitoprot.html)., the tccyps identified as with the highest probability to be localized to the parasite mitochondrion were aligned with human cypd protein sequence (genbankacc no. p30405) using […]

2014
PMCID: 4301749
PMID: 25479903
DOI: 10.1186/1471-2164-15-1069

[…] for fungal proteins []. for a query protein sequence, this server gives a relative score to each sub-cellular location and the highest scoring compartment has been used here to annotate aarss. mitoprot [] and tagetp [] servers were used to identify signal sequences within the predicted mitochondrial aarss. sequence alignment of aarss was performed using t-coffee program with default […]

2013
PMCID: 3572067
PMID: 23418572
DOI: 10.1371/journal.pone.0056465

[…] issued by the great barrier reef marine park authority., the alignment was generated using the clustalw and jalview programs. the presence of a mitochondrial target signal was predicted using the mitoprot program . protein disorder was predicted using three largely orthogonal methods, the iupred , disopred2 , and pondr vsl2 programs. for disorpred2 and pondr vsl2 the default cutoff value […]

2013
PMCID: 3591413
PMID: 23505474
DOI: 10.1371/journal.pone.0058261

[…] similarity study with other known sequences by use of clustalw available in bioedit software. intracellular localization prediction for all the sequences was done by using online tool wolfpsort or mitoprot ., for the analysis of band intensity in western blots or of fluorescence values in the caspase-3 assay, the t-test was used with p≤0.05 as significance level. sem ± was used in microsoft […]


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MITOPROT in publications

 (298)
PMCID: 5919513
PMID: 29698456
DOI: 10.1371/journal.pone.0196474

[…] 500,000) []., putative mitochondrial pre-sequences and the cleavage sites of mpp were predicted by gavel’s consensus patterns search in the psortii program (http://psort.hgc.jp/form2.html) [,] and mitoprot ii [], using the t. brucei mitoproteome dataset []. proteins with predicted pre-sequences were further analyzed for the mip cleavage motif (f/l/i)xx(t/s/g)xxxx [] within the octapeptide […]

PMCID: 5876329
PMID: 29599527
DOI: 10.1038/s41598-018-23690-y

[…] proposed role in nuclear dna repair, exd2 location is predicted to be mitochondrial/cytoplasmic. cellular and mitochondrial localization prediction programs vary in their estimation. for example mitoprot ii gives a reasonably high mitochondrial probability score of 69%, psort ii gives a poor mitochondrial prediction and targetp suggests the protein is secreted. several published papers […]

PMCID: 5844879
PMID: 29523818
DOI: 10.1038/s41598-018-22547-8

[…] pathway is thus conserved between human and yeast cells., tdp1 does not contain a canonical mitochondria-targeting presequence identifiable by mitochondrial targeting sequence predictors targetp, mitoprot and mitofates (data not shown). additionally, serial truncations from either the n- or c-terminus of tdp1 did not identify a distinct mitochondrial-targeting signal; rather they identified […]

PMCID: 5955198
PMID: 29261004
DOI: 10.1080/21505594.2017.1414133

[…] the cells is important for different aspects of virulence [,]., using bioinformatics, predictions can be made about the localization of nuclear encoded genes. for instance, we used psort ii [] and mitoprot [] on the predicted amino acid sequences of proteins of c. albicans that are required for virulence (). more than 70 proteins potentially have some level of mitochondrial localization, […]

PMCID: 5833341
PMID: 29467464
DOI: 10.1038/s41419-018-0365-8

[…] encoded by cg2658 contains an aaa domain (amino acid residues 377–513) and an m41 metallopeptidase domain (amino acid residues 575–785) acting as a proteolytic center (supplemental figure ). the mitoprot algorithm predicts that the n-terminal region of paraplegin contains a mitochondrial targeting sequence, and a proteomic proximity ligation study detected peptides corresponding to cg2658 […]


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MITOPROT institution(s)
Ecole Normale Supérieure, Laboratoire de Génétique Moléculaire, CNRS URA 1302, Paris, France
MITOPROT funding source(s)
Supported by a PFPI postdoctoral fellowship from the Ministerio de Education y Ciencia from Spain.

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