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Protocols specifications


Unique identifier OMICS_12758
Alternative name Multi Locus Sequence Typing Network
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Allowed
Maintained Yes


  • person_outline David M. Aanensen

Publication for Multi Locus Sequence Typing Network citations


A prospective cohort study of Staphylococcus aureus and methicillin resistant Staphylococcus aureus carriage in neonates: the role of maternal carriage and phenotypic and molecular characteristics

PMCID: 5926071
PMID: 29731644
DOI: 10.2147/IDR.S157522

[…] s sequence typing (MLST) PCR assays were performed by using previously published primers and conditions. Allelic profiles and sequence types (STs) were assigned by using the MLST database ( Singletons or members of a clonal complex (CC) were determined by using the eBURST algorithm ( A dendrogram analysis was performed based on ST types to determine the […]


Molecular Epidemiology of Staphylococcus epidermidis Implicated in Catheter Related Bloodstream Infections at an Academic Hospital in Pretoria, South Africa

Front Microbiol
PMCID: 5845871
PMID: 29563910
DOI: 10.3389/fmicb.2018.00417

[…] housekeeping genes (arcC, aroE, gtr, mutS, pyrR, tpi, and yqiL) was done by Inqaba Biotechnical Industries (Pretoria, South Africa). Originally, the S. epidermidis MLST database ( was used, [hosted now by pubMLST (] provided allelic profiles and the subsequent STs. A comparative electronic Based Upon Related Sequence Types (eBURST) v […]


Genomic differences between nasal Staphylococcus aureus from hog slaughterhouse workers and their communities

PLoS One
PMCID: 5839586
PMID: 29509797
DOI: 10.1371/journal.pone.0193820

[…] everal previously undetermined isolates were characterized here using srst2 [], which employs bowtie2 [] to call loci directly from Illumina reads based on the S. aureus MLST database ( spa types of all the isolates were determined by identifying spa repeats ( in the assemblies using BLAST [], followed by manual verification. […]


Virulence gene profiles: alpha hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

PMCID: 5834907
PMID: 29499697
DOI: 10.1186/s12917-018-1374-7

[…] ttp:// using SPATypeMapper software (download at and multilocus sequence typing (MLST; [, ]. In addition, the goeBURST algorithm ( was used to infer evolutionary associations of MLST types (STs). […]


Phylogenomic Based Comparative Studies on Indian and American Commensal Staphylococcus epidermidis Isolates

Front Microbiol
PMCID: 5835047
PMID: 29535698
DOI: 10.3389/fmicb.2018.00333
call_split See protocol

[…] omes were re-assembled in Spades version 3.11 (Bankevich et al., ), required genes were extracted and ST was analyzed. Generated STs were confirmed in MLST database (Aanensen and Spratt, ; Novel allele profile was submitted to PubMLST database (Jolley et al., ; to obtain the unique and novel ST information. […]


The Changing Pattern of Population Structure of Staphylococcus aureus from Bacteremia in China from 2013 to 2016: ST239 030 MRSA Replaced by ST59 t437

Front Microbiol
PMCID: 5835333
PMID: 29535697
DOI: 10.3389/fmicb.2018.00332
call_split See protocol

[…] NA was prepared using DNA Extraction Kit (TIANGEN Biotech, China). MLST was performed as described previously and the sequences of the PCR products were compared with an MLST database ( (Enright et al., ); a neighbor-joining tree was constructed with the concatenated sequences of the seven MLST genes (arcc, aroe, glpf, gmk, pta, tpi, and yqil) using MEGA5 (Tamura et al., ), […]


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Department of Infectious Disease Epidemiology, Imperial College London St Mary's Hospital, London, UK reviews

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