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MLST.net specifications

Information


Unique identifier OMICS_12758
Name MLST.net
Alternative name Multi Locus Sequence Typing Network
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Allowed
Maintained Yes

Maintainer


  • person_outline David M. Aanensen

Publication for Multi Locus Sequence Typing Network

MLST.net citations

 (335)
library_books

A prospective cohort study of Staphylococcus aureus and methicillin resistant Staphylococcus aureus carriage in neonates: the role of maternal carriage and phenotypic and molecular characteristics

2018
PMCID: 5926071
PMID: 29731644
DOI: 10.2147/IDR.S157522

[…] s sequence typing (MLST) PCR assays were performed by using previously published primers and conditions. Allelic profiles and sequence types (STs) were assigned by using the MLST database (http://www.mlst.net). Singletons or members of a clonal complex (CC) were determined by using the eBURST algorithm (http://eburst.mlst.net). A dendrogram analysis was performed based on ST types to determine the […]

library_books

Molecular Epidemiology of Staphylococcus epidermidis Implicated in Catheter Related Bloodstream Infections at an Academic Hospital in Pretoria, South Africa

2018
Front Microbiol
PMCID: 5845871
PMID: 29563910
DOI: 10.3389/fmicb.2018.00417

[…] housekeeping genes (arcC, aroE, gtr, mutS, pyrR, tpi, and yqiL) was done by Inqaba Biotechnical Industries (Pretoria, South Africa). Originally, the S. epidermidis MLST database (http://sepidermidis.mlst.net/) was used, [hosted now by pubMLST (https://pubmlst.org/sepidermidis/)] provided allelic profiles and the subsequent STs. A comparative electronic Based Upon Related Sequence Types (eBURST) v […]

library_books

Genomic differences between nasal Staphylococcus aureus from hog slaughterhouse workers and their communities

2018
PLoS One
PMCID: 5839586
PMID: 29509797
DOI: 10.1371/journal.pone.0193820

[…] everal previously undetermined isolates were characterized here using srst2 [], which employs bowtie2 [] to call loci directly from Illumina reads based on the S. aureus MLST database (http://saureus.mlst.net). spa types of all the isolates were determined by identifying spa repeats (http://spa.ridom.de) in the assemblies using BLAST [], followed by manual verification. […]

library_books

Virulence gene profiles: alpha hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

2018
PMCID: 5834907
PMID: 29499697
DOI: 10.1186/s12917-018-1374-7

[…] ttp://spa.ridom.de/index.shtml) using SPATypeMapper software (download at http://www.clondiag.com/fileadmin/Media/Downloads/SPATypeMapper_0_6.zip) and multilocus sequence typing (MLST; http://saureus.mlst.net/) [, ]. In addition, the goeBURST algorithm (http://goeBURST.phyloviz.net) was used to infer evolutionary associations of MLST types (STs). […]

call_split

Phylogenomic Based Comparative Studies on Indian and American Commensal Staphylococcus epidermidis Isolates

2018
Front Microbiol
PMCID: 5835047
PMID: 29535698
DOI: 10.3389/fmicb.2018.00333
call_split See protocol

[…] omes were re-assembled in Spades version 3.11 (Bankevich et al., ), required genes were extracted and ST was analyzed. Generated STs were confirmed in MLST database (Aanensen and Spratt, ; http://www.mlst.net). Novel allele profile was submitted to PubMLST database (Jolley et al., ; http://pubmlst.org/sepidermidis/) to obtain the unique and novel ST information. […]

call_split

The Changing Pattern of Population Structure of Staphylococcus aureus from Bacteremia in China from 2013 to 2016: ST239 030 MRSA Replaced by ST59 t437

2018
Front Microbiol
PMCID: 5835333
PMID: 29535697
DOI: 10.3389/fmicb.2018.00332
call_split See protocol

[…] NA was prepared using DNA Extraction Kit (TIANGEN Biotech, China). MLST was performed as described previously and the sequences of the PCR products were compared with an MLST database (http://saureus.mlst.net) (Enright et al., ); a neighbor-joining tree was constructed with the concatenated sequences of the seven MLST genes (arcc, aroe, glpf, gmk, pta, tpi, and yqil) using MEGA5 (Tamura et al., ), […]

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MLST.net institution(s)
Department of Infectious Disease Epidemiology, Imperial College London St Mary's Hospital, London, UK

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