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μManager specifications

Information


Unique identifier OMICS_06500
Name μManager
Alternative name Micro-Manager
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Publication for Micro-Manager

μManager citations

 (139)
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Toxicity of Food Grade TiO2 to Commensal Intestinal and Transient Food Borne Bacteria: New Insights Using Nano SIMS and Synchrotron UV Fluorescence Imaging

2018
Front Microbiol
PMCID: 5928251
PMID: 29740421
DOI: 10.3389/fmicb.2018.00794
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[…] nd 420–480 nm (Filter II, OMEGA Filters, United States) during 90 s of integration time. In addition, to increase the signal to noise ratio, images were recorded with binning of the pixels (2 × 2) in μManager software (), used to control the whole setup. For each condition, two independent quartz coverslips with at least three different locations, covering a minimum of 200 bacterial cells, were in […]

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USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability

2018
Cell Rep
PMCID: 5917450
PMID: 29669289
DOI: 10.1016/j.celrep.2018.03.100
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[…] omycin. Cells were imaged in a heated chamber (37°C) using an EC Plan-Neofluar 10×/0.3 objective on a Zeiss Axiovert 200M microscope. Images were acquired using a C4742-80-12AG camera (Hamamatsu) and μmanager software and were processed using ImageJ Fiji.For time-lapse analysis of duration of mitotic arrest in U2OS cells partially depleted of APC11 or APC2 and co-depleted of USP9X, cells were tran […]

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Different Membrane Pathways Mediate Ca2+ Influx in Adrenal Chromaffin Cells Exposed to 150–400 ns Electric Pulses

2018
Biomed Res Int
PMCID: 5896273
DOI: 10.1155/2018/9046891
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[…] nce images of the cells were captured before, during, and after stimulus application by an iXonEM + DU-897 EMCCD camera (Andor Technology, Ltd., Belfast, UK) using the open source microscopy software Micro-Manager (version 1.4, Vale Lab, UCSF, San Francisco, CA). The exposure time of the camera was set to 100 ms and images were captured at a rate of 7.5 Hz. Continuous baseline Ca2+ fluorescence of […]

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Compression force sensing regulates integrin αIIbβ3 adhesive function on diabetic platelets

2018
Nat Commun
PMCID: 5852038
PMID: 29540687
DOI: 10.1038/s41467-018-03430-6
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[…] n. Platelet adhesion was monitored using an inverted Leica DMIRB microscope and differential interference contrast (DIC) microscopy (×63, NA 1.2 water objective). Platelet adhesion was recorded using Micro-manager v1.4 for off-line analysis. For perfusion studies with human whole blood, mouse washed platelets or reconstituted blood, platelets were perfused through FGN-coated microslides (100 μg mL […]

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Simple and Inexpensive Paper Based Astrocyte Co culture to Improve Survival of Low Density Neuronal Networks

2018
Front Neurosci
PMCID: 5835045
PMID: 29535595
DOI: 10.3389/fnins.2018.00094

[…] with an EM-CCD camera (Hamamatsu C9100-13, Hamamatsu Photonics K. K., Switzerland) on an Axio Observer Z1 inverted microscope equipped with a Colibri LED light source (Carl Zeiss, Switzerland) using μManager (Edelstein et al., ).For image processing of astrocyte paper cultures, the number of live and dead cells was manually counted, and the three separate images per sample were summed. Due to sli […]

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AP2σ Mutations Impair Calcium Sensing Receptor Trafficking and Signaling, and Show an Endosomal Pathway to Spatially Direct G Protein Selectivity

2018
Cell Rep
PMCID: 5792449
PMID: 29420171
DOI: 10.1016/j.celrep.2017.12.089
call_split See protocol

[…] n TE2000 inverted microscope. Cells were perfused with extracellular bath solution with increasing CaCl2 concentrations. Fura-2 images were acquired using 340/380 nm excitation and 510 nm emission on μManager software (NIH). Methods for TIRF microscopy were adapted from previous studies (, ). Images were obtained with an Olympus IX-81 TIRF microscope. To monitor CaSR internalization, cells were pr […]

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μManager institution(s)
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA

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