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MMAPPR | Mutation mapping analysis pipeline for pooled RNA-seq

Maps mutations generated from forward genetic screens or that spontaneously arise in a population. MMAPPR can identify candidate mutations without any parental strain or genotype information, without previously identified single nucleotide polymorphism (SNP) map databases, and without data from separate individuals. By using only single RNA-seq libraries from a small number of pooled mutant individuals and their phenotypically wild-type siblings, it requires few animals and less sequencing data than required for whole-genome sequence mapping.

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MMAPPR classification

MMAPPR specifications

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Command line interface
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Restrictions to use:
Academic or non-commercial use
Programming languages:
Python, R

MMAPPR distribution


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Department of Neurobiology and Anatomy, University of Utah Molecular Medicine Program, University of Utah, Salt Lake City, UT, USA

Funding source(s)

Supported by a NHLBI Bench-to-Bassinet Consortium grant (U01HL0981) and a core facilities support grant to New England Research Institute (U01 HL098188).

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