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Mojo Hand specifications

Information


Unique identifier OMICS_04703
Name Mojo Hand
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for Mojo Hand

Mojo Hand in publications

 (9)
PMCID: 5936060
PMID: 29535102
DOI: 10.1242/bio.031096

[…] mutation, as done previously for fresh tissue (). to facilitate this, mutations can be designed to target restriction enzyme recognition sites. in fact, an online talen and crispr/cas9 design tool mojo hand () readily provides restriction enzyme sites targeted by a desired mutation. while newly emerging alternatives to standard pcr and rflp may provide a higher speed of genotyping (; ), […]

PMCID: 5756047
PMID: 29304078
DOI: 10.1371/journal.pone.0190897

[…] putative homologues of the fatty acid amide hydrolase 2 serine-serine-lysine catalytic triad [,]. the talens were designed to recognize sites conserved in all predicted splice variants by using the mojo hand version 2 software available online at http://www.talendesign.org []. the cnr1 talen binding sites were positioned around a 15 base pair spacer sequence containing a bstui restriction […]

PMCID: 5526933
PMID: 28743881
DOI: 10.1038/s41467-017-00143-0

[…] gene tools was used., crispr col12a1a and col12a1b grna were designed using a combination of different webtools: zifit (http://zifit.partners.org/zifit), crispr design (http://crispr.mit.edu), and mojo hand (http://talendesign.org). grna expression vectors were built by ligation of annealed oligonucleotides into pt7-grna expression vector (addgene #46759) as described previously. capped sense […]

PMCID: 5511212
PMID: 28710417
DOI: 10.1038/s41598-017-05696-0

[…] shown in hela cell line where a complete abrogation of e7 expression was observed. tools used to design talens aid researchers in targeting specific regions of the gene. while talen-t, e-talen and mojo hand design talens based on genomic context, restriction enzymes and off- target effects, sapta predicts talen activity based on rvd composition and site length, restriction enzymes. hence […]

PMCID: 5886277
PMID: 28934389
DOI: 10.1093/hmg/ddx255

[…] used at 1:400. alexa fluor 635 phalloidin (invitrogen) was used at 1:40. cell counting was done in imaris (bitplane) with manual threshold adjustment., target sites for talens were identified using mojo hand () and tale-nt 2.0 (). all sites chosen had a restriction enzyme site at the cut site to be used for genotyping (, table s4). talens were cloned and injected into one-cell zebrafish embryos […]


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Mojo Hand institution(s)
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Chinahester, MN, USA

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