Allows users to extract three-dimensional (3D) displacement and force information for motile cells. The 3D TFM methodology can be used for investigating the dynamic evolution of traction forces during migration of 3T3 fibroblasts on polyacrylamide substrates. It shows the viability of capturing cell-generated deformations in all three spatial dimensions with submicron accuracy. It enables the direct determination of three-dimensional displacement fields and direct calculations of traction forces using principles of mechanics.
A metrology toolbox for SFM, specifically aimed at biomolecules like DNA and proteins, which features (a) semi-automatic high-throughput analysis of individual molecules; (b) ease of use working within MATLAB environment or as a stand-alone application; (c) compatibility with MultiMode (Bruker), NanoWizard (JPK instruments), Asylum (Asylum research), ASCII, and TIFF files, that can be adjusted with minor modifications to other formats. Assembled in a single user interface, SFMetrics serves as a semi-automatic analysis tool capable of measuring several geometrical properties (length, volume and angles) from DNA and protein complexes, but is also applicable to other samples with irregular shapes.
Allows analysis of force microscopy recording like images or force curves. AtomicJ measures Young’s modulus, adhesion force, sample height and transition indentation. Users can produce two-dimensional maps of the mechanical properties and three-dimensional stacks. This software includes several different contacts models such as models for thin and hyperelastic samples, for blunt tips, the exact model for the sphere and models of adhesive contact.
A modular, multiplatform, open-source software for scanning probe microscopy data processing. Gwyddion is a fully functional software package can be created that covers the needs of a large part of the scanning probe microscopy user community.
Allows users to proceed to surface imaging, analysis and metrology. MountainsMap permits to display 3D surface topography and multi-channel image overlays in real time. It offers a way to manipulate all image layers simultaneously, zooming in on them, rotating them to any angle, and extracting profiles between the same points on each layer. This tool gathers options to scan anomalous lines and to measure artifacts.
A version of the public domain image analysis software NIH Image that has been extended to handle the loading, display and analysis of scanning microscope images. Image SXM supports SAM, SCM, SEM, SFM, SLM, SNOM, SPM and STM images.
Reconstructs traction forces of cells adhered on elastic gel doped with beads. TFM quantifies the deformation of the gel by image-based tracking of the beads from pairs of images, one displaying in the deformed and displaying the relaxed substrate. It can be applied for identifying traction from small adhesions and generates traction magnitude heat-maps as well as traction vector fields.
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