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Quantifying cell morphology in bright field images yields the possibility to measure more descriptive features such as texture and shape simultaneously. In addition, the fluorescence staining of e.g. the cytoplasm could be incomplete or less reliable on a special cell type, which would introduce bias in the later analysis.
(Buggenthin., 2013) An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy. BMC Bioinformatics.