Finds dynamic changes in pixel intensity between image frames. MUSCLEMOTION expresses the output as a relative measure of movement during muscle contraction and relaxation. It can be employed for multiparameter recording conditions and experimental settings using transmitted light microscopy, fluorescent membrane labeling, fluorescent beads embedded in soft substrates or patch clamp video recordings.
Serves for effective segmentation of multidimensional datasets. MIB can recognize several number of imaging formats and offers a variety of image processing tools. It also simplifies utilization and quantification of acquired data. It permits users to segment large datasets, to realize 3D visualization, and to quantify images and models. Its parameters enable users to insert plugin s to customize the program for specific needs.
Enables cell lineage tracking. MicrobeTracker utilizes cell shape and timelapse information to achieve cell outlining. It can track fluorescently labeled molecules in cell lineages over several generations or in difficult-to-resolve samples, such as densely-packed or filamentous cells, from time-lapse sequences. This tool is delivered with an accessory tool, called SpotFinder, that detects small round spots, generating precise cell coordinates of fluorescently labeled foci inside cells.
Permits analyses of microscopy images. SarcOptiM is available through an online mode that allows real-time analysis and display of the contraction of a cell along a line drawn along its longitudinal axis. It also provides an offline version dedicated to the analysis of pre-recorded videos of contracting isolated cardiomyocyte (CM). This tool can be used for adjusting brightness or contrast, defining areas of interest, or for other processing on images or videos.
Allows for quantitative assessment of morphology and pericyte coverage in vascular sprouts developed from human endothelial cells and human pericytes in vitro. Sprout Morphology enables to observe some of the expected effects of anti-angiogenic substances, such as those of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) receptor inhibitors. It is a plugin for ImageJ allowing to quantify the results.
Provides an image analysis software for prokaryotes with complex morphologies. BacStalk is an application specialized for automated, label-free, time-resolved image analysis of stalked and non-stalked bacteria. It enables the detection of fluorescence patterns and the morphometric analysis of cell bodies, and their corresponding stalks and buds. It can also visualize fluorescence signals in cells in interactive demographs and kymographs.
Marks and links results in a non-destructive way. ObjectJ uses vector objects to display points and paths that non-destructively mark images via a transparent layer. This ImageJ plugin stores the graphical objects into a separate project file. A back-and-forth navigation between results and images is available and the results table can incorporate statistics, sorting, color coding, qualifying and macro access.
Serves as a cell analysis tool package. DucoCell allows users to count cells and evaluate images for morphological parameters of cells such as area, perimeter, diameter, ellipticity or form-factor. The software permits simultaneous and automatic analysis of multiple images, and then export results and create a result graph. It also provides a capture feature that enables users to collect single-frame images and time-lapse experiments.
Allows to visualize and present your images in several dimensions. The functionality of this imaging toolbox expands constantly with a wide range of different modules that are tailored to specific applications or microscope accessories. AxioVision offers countless functions for applications in the field of biological and medical routine research.
Offers a platform for fractal analysis and morphology functions. FracLac is a module that can be used as an ImageJ or FIJI plugin. The application includes features such as: (i) analyzing complexity and heterogeneity, (ii) measuring difficult to describe geometrical forms; (iii) extracting of pattern from several types of images for analysis. The software is suitable for images of biological cells as well as for other structures such as branching structures or known fractals.
Allows users to determine Fourier descriptors and the curvature starting from a closed curve. Fourier Shape analysis is a module that can be run through the ImageJ software. The application consists of three main features allowing the computation of Fourier descriptors, the generation of elliptic Fourier descriptors including a normalized set of coefficients such as rotation or scale invariant, as well as the calculation of curvature.
Allows classification of HEp-2 cell images. RSurf is a texture-based image descriptor that computes the properties, such as height and length, of each slope (i.e. a 1D sequence of neighboring pixels, following the straight line, where pixel intensities change monotonously) in the 2D image and derives statistics of them. It includes a sample image allowing to test the software.
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