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MotionCorr specifications

Information


Unique identifier OMICS_17200
Name MotionCorr
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
License GNU General Public License version 3.0
Computer skills Advanced
Version 2.1
Stability Stable
Maintained No

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Publication for MotionCorr

MotionCorr citations

 (105)
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Structural and immunologic correlates of chemically stabilized HIV 1 envelope glycoproteins

2018
PLoS Pathog
PMCID: 5944921
PMID: 29746590
DOI: 10.1371/journal.ppat.1006986
call_split See protocol

[…] Movie micrograph frames were aligned using MotionCorr [] and CTF models were calculated using CTFFIND3 []. The resulting motion-corrected and signal-integrated micrographs were subjected to automated difference-of-Gaussian particle selection [ […]

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Structural basis for cofilin binding and actin filament disassembly

2018
Nat Commun
PMCID: 5945598
PMID: 29749375
DOI: 10.1038/s41467-018-04290-w
call_split See protocol

[…] A total of 1,111 images were collected in two microscope sessions (414 and 697 micrographs, respectively). Image processing was performed with RELION 2.0 software,. After motion correction with MotionCorr and CTF estimation with Gctf, 1,093 images were selected for further image processing. Filaments were manually picked with e2helixboxer, after which 245,236 particles were extracted at a bo […]

library_books

RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co chaperone complex

2018
Nat Commun
PMCID: 5902453
PMID: 29662061
DOI: 10.1038/s41467-018-03942-1

[…] As general methodology, MotionCorr2 was used for whole-frame motion correction, GCTF for estimation of the contrast transfer function parameters, and RELION-2.0 for all subsequent steps. Local motion was corrected in MotionC […]

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Cryo EM structure of the polycystic kidney disease like channel PKD2L1

2018
Nat Commun
PMCID: 5864754
PMID: 29567962
DOI: 10.1038/s41467-018-03606-0
call_split See protocol

[…] by Jianlin Lei). For each micrograph stack, 38 frames were collected, with a total electron dose at approximately 60 e-/Å2 with an exposure time of 2.25 s. The stacks were first motion corrected with MotionCorr and the output stacks were further motion corrected with MotionCor2. Dose weighting was performed concurrently. Gctf was used to estimate the defocus values. […]

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Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa

2018
PMCID: 5807055
PMID: 29307484
DOI: 10.1016/j.str.2017.12.005
call_split See protocol

[…] 0-2.5 μm using FEI EPU software for automated data-collection. A total of 1,405 “movie” mode image stacks were obtained. These image stacks were corrected for the effects of beam-induced motion using MOTIONCORR (). Motion-corrected sum images were produced using all recorded frames. Image Contrast Transfer Function (CTF) was determined by Gctf (), which enabled the selection of 1,280 out of 1,405 […]

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Development of a yeast internal subunit eGFP labeling strategy and its application in subunit identification in eukaryotic group II chaperonin TRiC/CCT

2018
Sci Rep
PMCID: 5799240
PMID: 29403048
DOI: 10.1038/s41598-017-18962-y
call_split See protocol

[…] Image processing and 3D reconstruction processes are listed in Table . Motion corrections were performed with Motioncorr before further image processing. CTF parameters of each image were determined with CTFFIND3. Particles were picked automatically with Relion1.3, and were further cleaned up through 2D class […]

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MotionCorr institution(s)
The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA; The Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA; Gatan Inc., Pleasanton, CA, USA
MotionCorr funding source(s)
This work is supported by the UCSF Program for Breakthrough Biomedical Research and NIH grants (R01GM082893, R01GM098672 and P50GM082250).

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