MPD statistics

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Popular tool citations

chevron_left Multiplex primer design Oligonucleotide design chevron_right
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MPD specifications

Information


Unique identifier OMICS_14711
Name MPD
Alternative name Multiplex Primer Design
Interface Web user interface
Restrictions to use None
Input data A list of coordinates to amplify
Input format BED
Output data The final primer design summary viewed in the browser
Programming languages Javascript, Perl
Computer skills Basic
Stability Stable
Registration required Yes
Maintained Yes

Documentation


Maintainer


  • person_outline Thomas Wingo <>

Additional information


A registration is needed to access to tools.

Information


Unique identifier OMICS_14711
Name MPD
Alternative name Multiplex Primer Design
Software type Package/Module
Interface Command line interface
Restrictions to use None
Input data A list of coordinates to amplify
Input format BED
Output data The final primer design summary
Operating system Unix/Linux, Mac OS, Windows
Programming languages C, Perl
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Source code URL https://codeload.github.com/wingolab-org/mpd-c/legacy.tar.gz/master
Maintained Yes

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Documentation


Maintainer


  • person_outline Thomas Wingo <>

Additional information


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Publication for Multiplex Primer Design

MPD in publications

 (2)
PMCID: 4668122
PMID: 26630669
DOI: 10.1371/journal.pone.0144349

[…] local stand alone blast using a database encompassing the nucleotides sequences of all 47 available c. jejuni capsule loci (c. t. parker, unpublished). the selected nucleotide regions were used for multiplex primer design. the sites where the primers were designed are displayed in . multiplex primers were designed via the online software primer3 [] using the following parameters: length […]

PMCID: 4031183
PMID: 24854517
DOI: 10.1371/journal.pone.0098341

[…] the downside of modular type of primers is their higher synthesis and purification cost. although not tested here, we believe these multiplexing methods can be further improved by implementing multiplex primer design strategies that minimize interactions . in addition we show that in some cases multiplexing can be effectively resolved by using primer pairs that each target more than one […]


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MPD institution(s)
Division of Neurology, Atlanta VA Medical Center, Decatur, GA, USA; Department of Neurology, Emory University School of Medicine, Atlanta, GA, USA; Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA
MPD funding source(s)
Supported by the Veterans Health Administration (BX001820), National Institutes of Health (AG025688), and the Emory Integrated Genomics Core (EIGC).

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