MPprimer specifications

Information


Unique identifier OMICS_02364
Name MPprimer
Alternative name Multiplex Polymerase primer
Software type Application/Script
Interface Command line interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux, Mac OS
Programming languages Python
License GNU General Public License version 3.0
Computer skills Advanced
Version 1.4
Stability Stable
Maintained Yes

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Documentation


Information


Unique identifier OMICS_02364
Name MPprimer
Alternative name Multiplex Polymerase primer
Interface Web user interface
Restrictions to use None
Input data A set of template DNA sequences
Input format FASTA
Output data Primer Set Combination
Programming languages Python
License GNU Lesser General Public License version 3.0
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Publication for Multiplex Polymerase primer

MPprimer in publications

 (10)
PMCID: 5785846
PMID: 29370841
DOI: 10.1186/s13041-018-0346-y

[…] to the frozen sample (total volume: 13 μl). next, rt was performed for 1 h at 37 °c. subsequently, a multiplex pcr was performed to identify transcripts of inhibitory neurotransmitter transporters. mpprimer software [] was used to create primer sequences (table ). primers were chosen to be located on different exons. thus, amplification of dna, which contains exons and introns, would result […]

PMCID: 5739048
PMID: 29302302
DOI: 10.1093/aobpla/plx060

[…] 4 (repeats between 4 and 20) and 184 on chromosome 5 (repeats between 4 and 11). several combinations of different ssrs were tested to find compatible multiplex pcr amplifications using the mpprimer software. the two sets of compatible multiplex pcr developed were based on: (i) ease of score, (ii) a compatible allele size range and (iii) similar optimal reaction conditions. three ssrs […]

PMCID: 5793476
PMID: 29355148
DOI: 10.4103/ijmr.IJMR_623_16

[…] factor (24b1) and hypothetical protein (8d6) was done using the available gene sequences (acc.nos. hm584933-hm584937). primers () were selected and designed using ncbi primer blast programme, and mpprimer design software was used for multiplex pcr primer design. the primers were synthesized commercially (sigma, usa) and the dna from both the standard strains were used for the multiplex pcr. […]

PMCID: 5363871
PMID: 28333958
DOI: 10.1371/journal.pone.0174265

[…] were designed using the multiplx online tool (http://bioinfo.ebc.ee/multiplx) and the resulting three primer mixes were tested for incompatibilities with pridimerchek (http://biocompute.bmi.ac.cn/mpprimer/primer_dimer.html). for target amplification, 1–2 ng of bisulfite treated cfdna were used in pcr reactions with the gotaq® hotstart dna polymerase (promega corporation) and with the program: […]

PMCID: 5355300
PMID: 28036300
DOI: 10.18632/oncotarget.14340

[…] excess ddntps were removed using shrimp alkaline phosphatase (affymetrix, santa clara, ca, usa) and analyzed using a 3130xl genetic analyzer (applied biosystems). primers for pcr were designed using mpprimer []. primers for sne were manually selected and screened using primer designer (v.5) software (scientific & educational software, morrisville, nc, usa). the sne primers were designed […]


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MPprimer institution(s)
Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing, China; Beijing Institute of Health Administration and Medical Information, Beijing, China
MPprimer funding source(s)
This work was supported by National Basic Research Project (2006CB504100), the National Key Technologies R&D Program for New Drugs (2009ZX09103-616, 2009ZX09503-002, 2009ZX09301-002), General Program (30900862, 30973107, 30771230) of General Program of National Natural Science Foundation of China, the State Key Laboratory of Proteomics (SKLP2010yx-005), Major Program for Science and Technology Research of Beijing Municipal Bureau (7061004).

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