MS-Bridge

[…] complex described above. the purified proteolytic fragments obtained after digestion, were characterized by spectroscopy and analyzed by mass spectrometry (ms) at the university of edinburgh. ms-bridge prospector platform (http://prospector.ucsf.edu) was used for search and identification. the elemental composition of modified cysteines and/or the chromophore as crosslinking agent […]

[…] tesque) as internal standards and with α-cyano-4-hydroxycinnamic acid (wako) as a matrix. spectra were obtained using ultraflex™ (bruker daltonics) in linear mode, and mass peaks were assigned using ms bridge (university of california, san francisco, ca)., it has been reported that agitation of apo-sod1s-s forms insoluble aggregates in the presence of 1 m gdn (chattopadhyay et al., ; toichi et […]

[…] for the mass range of 700–3500 amu. data was collected and analyzed using the data explorer software package (ab-sciex, framingham, ma) and mascot (ver.2.0.04, matrix science, boston, ma). ms-bridge analysis was done using protein prospector (ver.4.27.2 basic and ver.5.0, ucsf). the database search parameters were: (i) tryptic digest, (ii) oxidized met, (iii) 2 missed cleavages, […]

[…] moieties for d0- (i.e., a, s, t) or d10-dmsso (i.e., a*, s*, t*) cross-linker, respectively. initial acceptance criteria for peptide identification required a reported expectation value ≤0.1., ms-bridge was used to confirm the identification of cross-linked peptides by mass mapping against bovine cytochrome c with the parent mass error set as ±10 ppm. the in-house program link-hunter […]

[…] regional proteomics facility. recovered peptides were subjected to matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry, and the results analyzed using the ucsf ms-bridge webpage. to delineate the boundary of the trypsin- and elastase-stable catalytic domain, liquid chromatography (lc) mass spectrometry was performed on a 3q mass spectrometer (micromass) […]