MTrackJ protocols

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MTrackJ specifications

Information


Unique identifier OMICS_06576
Name MTrackJ
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Erik Meijering <>

MTrackJ in pipelines

 (2)
2016
PMCID: 5164929
PMID: 27750279
DOI: 10.1038/nature19801

[…] are available upon request. distance measurements from this segmentation were performed as described in. cell tracking was performed using imaris (bitplane, switzerland) and the fiji plugin mtrackj. for accuracy in cell tracking data, videos were registered when required before using 4d data protocols implemented in fiji. 3d data rendering and measurement of cell division distances […]

2015
PMCID: 4529032
PMID: 26116662
DOI: 10.1242/dev.122473

[…] resolution of 30 min/time point. translational and rotational drift correction in the xy plane was performed using control point registration in matlab. cell tracking was performed manually using mtrackj in fiji (), where the point of the cell soma closest to the basal surface of the retina was selected. to calculate radial and tangential movements of cells, a spherical coordinate system […]

MTrackJ in publications

 (301)
PMCID: 5955935
PMID: 29769531
DOI: 10.1038/s41467-018-04284-8

[…] of fluorescent beads was performed using a custom-made imagej script (available as supplementary software 1 file). the macro generates an automated tracking file that is compatible with the imagej mtrackj plugin. following automated detection, tracks were manually curated using mtrackj to correct for any errors of the tracking algorithm and the tracking parameters exported in a .csv datasheet […]

PMCID: 5913241
PMID: 29707230
DOI: 10.1038/s41522-018-0052-7

[…] using a ×20 elwd objective. a stack of at least 150 frames (100 ms exposure time) was recorded for each sample and the movement of 15 randomly sampled motile cells per stack was traced using the mtrackj plugin in comstat. the number of frames a cell spent orbiting (o) within a 10 × 10 μm2 area was divided by the total number of frames (t) to give o/t. data from three stacks of three […]

PMCID: 5912900
PMID: 29606421
DOI: 10.1016/j.cub.2018.02.048

[…] camera (du 897 ixon ultra (andor)). frame rates were 0.5/s and 1/s for mitochondria and dcvs, respectively. image series were captured for 3 min. tracking of mitochondria was performed manually (mtrackj) on the raw movies by marking the start and end of each run. tracking was performed blind to the experimental condition or genotype using the randomizer macro (tiago ferreira, mcgill […]

PMCID: 5841660
PMID: 29513666
DOI: 10.1371/journal.pone.0192497

[…] axiovert 200m microscope endowed with a live-cell heating chamber. pictures were taken at 20x magnification every 9 minutes over a total time of 3 hours, and analyzed with imagej using the plug-in mtrackj. to assess the effects of the lrrk2 inhibitor czc-25146 on outgrowing neurites, neurons were pre-treated with the compound before picking, and subsequently transferred to czc-25146 containing […]

PMCID: 5878195
PMID: 29508172
DOI: 10.1007/s11255-018-1831-z

[…] using the image analysis software imagej (rasband, w.s., imagej, us national institutes of health, bethesda, maryland, usa, http://imagej.nih.gov/ij/, 1997–2014) and the cell tracking plugin mtrackj (created by erik meijering; www.imagescience.org/meijering/software/mtrackj). five cells per film were selected and tracked frame by frame in order to measure the mean velocity […]

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