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Protocols

MTrackJ specifications

Information


Unique identifier OMICS_06576
Name MTrackJ
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Erik Meijering

MTrackJ citations

 (314)
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Myosin1D is an evolutionarily conserved regulator of animal left–right asymmetry

2018
Nat Commun
PMCID: 5955935
PMID: 29769531
DOI: 10.1038/s41467-018-04284-8
call_split See protocol

[…] ng of fluorescent beads was performed using a custom-made ImageJ script (available as Supplementary Software 1 file). The macro generates an automated tracking file that is compatible with the ImageJ MTrackJ plugin. Following automated detection, tracks were manually curated using MTrackJ to correct for any errors of the tracking algorithm and the tracking parameters exported in a .CSV datasheet f […]

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A Tad pilus promotes the establishment and resistance of Vibrio vulnificus biofilms to mechanical clearance

2018
PMCID: 5913241
PMID: 29707230
DOI: 10.1038/s41522-018-0052-7
call_split See protocol

[…] cope using a ×20 ELWD objective. A stack of at least 150 frames (100 ms exposure time) was recorded for each sample and the movement of 15 randomly sampled motile cells per stack was traced using the MTrackJ plugin in Comstat. The number of frames a cell spent orbiting (O) within a 10 × 10 μm2 area was divided by the total number of frames (T) to give O/T. Data from three stacks of three biologica […]

library_books

A cAMP/PKA/Kinesin 1 Axis Promotes the Axonal Transport of Mitochondria in Aging Drosophila Neurons

2018
Curr Biol
PMCID: 5912900
PMID: 29606421
DOI: 10.1016/j.cub.2018.02.048

[…] -CCD camera (Du 897 iXon Ultra (Andor)). Frame rates were 0.5/s and 1/s for mitochondria and DCVs, respectively. Image series were captured for 3 min. Tracking of mitochondria was performed manually (MTrackJ) on the raw movies by marking the start and end of each run. Tracking was performed blind to the experimental condition or genotype using the Randomizer macro (Tiago Ferreira, McGill Universit […]

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Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease

2018
PLoS One
PMCID: 5841660
PMID: 29513666
DOI: 10.1371/journal.pone.0192497
call_split See protocol

[…] an Axiovert 200M microscope endowed with a live-cell heating chamber. Pictures were taken at 20x magnification every 9 minutes over a total time of 3 hours, and analyzed with ImageJ using the plug-in MTrackJ. To assess the effects of the LRRK2 inhibitor CZC-25146 on outgrowing neurites, neurons were pre-treated with the compound before picking, and subsequently transferred to CZC-25146 containing […]

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Epidermal growth factor receptor function in the human urothelium

2018
PMCID: 5878195
PMID: 29508172
DOI: 10.1007/s11255-018-1831-z
call_split See protocol

[…] analyzed using the image analysis software ImageJ (Rasband, W.S., ImageJ, US National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2014) and the cell tracking plugin MTrackJ (created by Erik Meijering; www.imagescience.org/meijering/software/mtrackj). Five cells per film were selected and tracked frame by frame in order to measure the mean velocity of these cells. […]

library_books

Neutrophil specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish

2018
PMCID: 5897731
PMID: 29590639
DOI: 10.1242/dmm.033027

[…] rophil motility were obtained by a laser scanning confocal microscope (LSM 710, Zeiss) with a 20× objective at 1-min intervals for 30 min. The velocity of neutrophils was quantified using ImageJ with MTrackJ plugin and plotted in Prism 6.0 (GraphPad). […]

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