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mTRIP specifications

Information


Unique identifier OMICS_23312
Name mTRIP
Alternative name Mitochondrial Transcription and Replication Imaging Protocol
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Miria Ricchetti

Publication for Mitochondrial Transcription and Replication Imaging Protocol

mTRIP citations

 (6)
library_books

Endonuclease G promotes mitochondrial genome cleavage and replication

2018
Oncotarget
PMCID: 5915074
PMID: 29719607
DOI: 10.18632/oncotarget.24822

[…] In order to analyse a potential role of EndoG in mtDNA replication and transcription at the single-cell level, we used the established mTRIP imaging protocol [–]. The principle of mTRIP is a combination of RNA and DNA fluorescence in situ hybridization (FISH), relying on the following probe design: the mREP probe is localised in the […]

library_books

Helicobacter pylori targets mitochondrial import and components of mitochondrial DNA replication machinery through an alternative VacA dependent and a VacA independent mechanisms

2017
Sci Rep
PMCID: 5698309
PMID: 29162845
DOI: 10.1038/s41598-017-15567-3

[…] uorescence staining and Mitotracker® Deep-Red (LifeTechnologies) mitochondria labelling, three-dimensional (3D) imaging acquisition and quantification and modified fluorescence in situ hybridization (mTRIP) are described in the Supporting information. […]

library_books

Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells

2017
Biophysics Rep
PMCID: 5719805
PMID: 29238743
DOI: 10.1007/s41048-017-0037-8

[…] Understanding the replication and transcription of mtDNA is important to a complete picture of mitochondrial dynamics. The mitochondrial transcription and replication imaging protocol (mTRIP) is a modified fluorescence in situ hybridization approach that specifically targets replicating mtDNA nucleoids and can simultaneou […]

library_books

Sepsis induces long term metabolic and mitochondrial muscle stem cell dysfunction amenable by mesenchymal stem cell therapy

2015
Nat Commun
PMCID: 4682118
PMID: 26666572
DOI: 10.1038/ncomms10145

[…] DNA probes for the fluorescence in situ hybridization protocol called Mitochondrial Transcription and Replication Imaging Protocol (mTRIP) was designed for the mouse mitochondrial genome and amplified using total mice DNA and the following sets of primers:mREP (coordina […]

library_books

Strength in numbers: quantitative single‐molecule RNA detection assays

2015
PMCID: 5024021
PMID: 25645249
DOI: 10.1002/wdev.170

[…] utilized by Santangelo et al. to tetramerize short 2′O‐methyl RNA oligos labeled with conventional fluorophores. A few (usually two or three) of these multiply labeled tetravalent RNA imaging probes (MTRIP) per target has been shown to provide sufficient brightness and contrast to detect individual β‐actin mRNAs upon streptolysin‐O‐mediated delivery into living cells. Another potential weakness of […]

library_books

Combining Single RNA Sensitive Probes with Subdiffraction Limited and Live Cell Imaging Enables the Characterization of Virus Dynamics in Cells

2013
ACS Nano
PMCID: 3906890
PMID: 24351207
DOI: 10.1021/nn405998v

[…] In addition to biochemical and plaque assays, we examined MTRIP-labeled virion replication in cell culture using fluorescence microscopy. After inoculating cells with MTRIP-labeled hRSV, we delivered MTRIPs with a different fluorophore and fixed the cells at […]

Citations

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mTRIP institution(s)
Team “Stability of nuclear and mitochondrial DNA”, Unité de Génétique Moléculaire des Levures, CNRS UMR3525, Institut Pasteur, Paris, France
mTRIP funding source(s)
Supported by the Association Nationale contre le Cancer (ARC 4022 and SFI20111204038), PTR-Institut Pasteur (PTR217), DARRI-Institut Pasteur (project P790319), Association Française contre les Myopathies (AFM, project 2012-0905 n° 16290), and Agence Nationale pour la Recherche (ANR 11BSV202502); a Bourse Roux Institut Pasteur and by M. François R. Lacoste.

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