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Multilocus sequence typing software tools | De novo genome sequencing data analysis

Accurate strain identification is essential for anyone working with bacteria. For many species, multilocus sequence typing (MLST) is considered the “gold standard” of typing, but it is traditionally performed in an expensive and time-consuming manner. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available to scientists and routine diagnostic laboratories.

Source text:
(Larsen et al., 2012) Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol.

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BIGSdb / Bacterial Isolate Genome Sequence Database
Allows storage and analysis of sequence data for bacterial isolates. BIGSdb is a software platform developed to host, flexibly organize, and extract allelic variants for any locus of interest. A Representational State Transfer (REST) API, developed as an integral component of the software, facilitates the exposure of all public data held within the site from any programming environment. It exists many instances of BIGSdb databases and it is used to host PubMLST resources.
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