Multipass protocols

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Multipass specifications


Unique identifier OMICS_11613
Name Multipass
Interface Web user interface
Restrictions to use None
Input data Raw sequencing data, mapping file (MID-tag and primer sequences for samples to be extracted from the sff-file and processed with Multipass)
Input format SFF, MAP
Output format ZIP
Biological technology Roche
Computer skills Basic
Version 1.0
Stability Stable
Maintained Yes


  • person_outline Thomas S. Rask <>

Publication for Multipass

Multipass in pipelines

PMCID: 5610382
PMID: 28414526
DOI: 10.1089/cmb.2017.0008

[…] v1.2.10, metavelvet v1.2.02, mira v4.0.2, spades v3.5.0, and omega v1.0.2. a brief summary of the assemblers is given below and the parameters used for each assembler are summarized in ., mira is a multipass dna sequence assembler that uses the olc and greedy algorithms to assemble prokaryote and small eukaryote genomes sequenced on most of the current sequencing technologies, within […]

PMCID: 4393059
PMID: 25937952
DOI: 10.1155/2013/962760

[…] cell's surface and plasma membrane. 14 proteins were identified as soluble proteins that include 7 secreted proteins, and 63 proteins were membrane proteins including 20 type i, 21 type ii, and 22 multipass membrane proteins ()., the ppi network of the 84 protein nodes was extracted from the overlapping ppi network in and presented in . this smaller ppi network of cell's surface and membrane […]

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Multipass in publications

PMCID: 5955956
PMID: 29769544
DOI: 10.1038/s41598-018-25783-0

[…] energy of 1.5 mj and a pulse duration of ~150 ps, whose beam pointing is actively stabilized by a beam stabilizer (aligna 4d, tem messtechnik), is further amplified to a pulse energy of 1 j by a multipass power amplifier (mpa). the mpa is pumped by two flash-lamp-pumped lasers (precision-ii, continuum) which are operated at a 10 hz repetition rate. after the mpa, a grating compressor, […]

PMCID: 5951517
PMID: 29677128
DOI: 10.3390/ma11040633

[…] bottom surfaces and compressive stress presenting in the interior. this kind of stress distribution is the common type of the as-welded transverse at the weld centerline for butt-welded joints by multipass arc welding with the single v groove or double v-type groove as demonstrated by kartal et al. [], liu et al. [,], and smith et al. []., the measured surface stresses by the xrd method […]

PMCID: 5917893
PMID: 29713685
DOI: 10.1126/sciadv.aaq1526

[…] high average power is important for experimental techniques with low yield such as stand-off gas detection () and may avoid the need for multiple interactions with the sample in resonant cavities or multipass cells ()., here, the issue of the scarce availability of high-power femtosecond sources in the mir is addressed by taking advantage of the most powerful laser architectures in the nir […]

PMCID: 5841281
PMID: 29515179
DOI: 10.1038/s41598-018-22487-3

[…] contrast associated with ase or pulses replicas on a time scale up to 0.5 ns prior to the main pulse, we use a third order autocorrelator (sequoia, amplitude technologies)., in the laser chain, a multipass amplifier (booster amplifier) is inserted in between the oscillator and the stretcher. the non-stretched beam is amplified up to a few µj per pulse and then it is temporally cleaned […]

PMCID: 5845867
PMID: 29563917
DOI: 10.3389/fimmu.2018.00460

[…] host (). this can be done by fusing the ecd to another well-characterized transmembrane domain or by adding a signal sequence for a c-terminal glycosylphosphatidylinositol (gpi)-anchor (, ). multipass membrane proteins that contain extracellular loops (ecls) present significant challenges for antigen engineering. attempts to utilize the isolated loop sequences as peptide immunogens […]

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Multipass institution(s)
Department of Systems Biology, Center for Biological Sequence Analysis, Technical University of Denmark, Kongens Lyngby, Denmark; Division of Medical Parasitology, Department of Microbiology, New York University Langone Medical Center, New York, NY, USA; School of Biosciences, The University of Melbourne, Parkville, Victoria, Australia
Multipass funding source(s)
This work was supported by the National Institute of Allergy and Infectious Disease, National Institutes of Health [grant number R01-AI084156]; the Fogarty International Center at National Institutes of Health [Program on the Ecology and Evolution of Infectious Diseases, grant number R01-TW009670]; and the Lundbeck Foundation [grant number R48-A4847].

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