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MASC | A Linear Method for Multiple Nucleotide Sequence Alignment on Spark Parallel Framework


Allows users to perform multiple sequences alignments (MSA) among highly similar sequences. MASC is a standalone software which can be applied for MSA in linear time and for very high-throughput cases. The method is implemented on Spark-distributed parallel framework for massive scale sequence data with the aim of accelerating the process. However, it has been noticed that the software decreases in efficiency when it must manage complex variations.

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MASC classification

MASC specifications

Unique identifier:
Software type:
Restrictions to use:
Computer skills:
Multiple sequence Alignment based on a Suffix tree and Center-star strategy
Command line interface
Operating system:

MASC distribution


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MASC support


  • Quan Zou <>
  • Shaoliang Peng <>


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School of Computer Science and Technology, National University of Defense Technology, Changsha, China; School of Computer Science and Technology, Tianjin University, Tianjin, China; College of Computer Science and Electronic Engineering, National Supercomputer Center in Changsha, Hunan University, Changsha, China

Funding source(s)

Supported by National Key R&D Program of China 2017YFB0202600, 2016YFC1302500, 2016YFB0200400, and 2017YFB0202104; NSFC Grants 61772543, U1435222, 61625202, and 61272056; and Guangdong Provincial Department of Science and Technology under grant no. 2016B090918122.

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.