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Multiple nucleotide sequence alignment evaluation software tools | High-throughput sequencing data analysis

Multiple sequence alignment (MSA) is the basis for a wide range of comparative sequence analyses from molecular phylogenetics to 3D structure prediction. Sophisticated algorithms have been developed for sequence alignment, but in practice, many errors can be expected and extensive portions of the MSA are unreliable. Hence, it is imperative to understand and characterize the various sources of errors in MSAs and to quantify site-specific alignment confidence.

Source text:
(Penn et al., 2010) An alignment confidence score capturing robustness to guide tree uncertainty. Mol Biol Evol.

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