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Multiplex primers design software tools | qPCR analysis

The goal of multiplex PCR is to amplify several targets in the same tube. Sets of primer pairs can be checked for cross-dimerization.

MSRE-HTPrimer
Desktop

MSRE-HTPrimer

Designs all possible PCR primer pairs and oligos, followed by filtering for…

Designs all possible PCR primer pairs and oligos, followed by filtering for single nucleotide polymorphisms (SNPs) loci and repeat regions. MSRE-HTPrimer is an open-source, portable, web-based use…

MPD
Web
Desktop

MPD Multiplex Primer Design

Automates the design of multiplex PCR primers for next-generation sequencing.…

Automates the design of multiplex PCR primers for next-generation sequencing. The core of MPD is implemented in C for speed and uses a hashed genome to ensure primer uniqueness, avoids placing…

EvOligo
Desktop

EvOligo

Offers time-efficient sorting of paired and unpaired oligonucleotides based on…

Offers time-efficient sorting of paired and unpaired oligonucleotides based on various parameters defined by the user. EvOligo is a software package that provides the means to design and group DNA…

AutoDimer
Web

AutoDimer

Developed to rapidly screen previously selected PCR primers for primer-dimer…

Developed to rapidly screen previously selected PCR primers for primer-dimer and hairpin interactions in short DNA oligomers.

PrimerStation
Web

PrimerStation

A web service to calculate optimal primer sets that are guaranteed high…

A web service to calculate optimal primer sets that are guaranteed high specificity against whole human genome.

MultiPLX
Web
Desktop

MultiPLX

Provides a tool for automatic grouping of polymerase chain reaction (PCR)…

Provides a tool for automatic grouping of polymerase chain reaction (PCR) primers for multiplexed PCR. MultiPLX performs grouping by calculating many important interaction levels between the…

TipMT
Web

TipMT Tool for Identification of Primers for Multiple Taxa

Identifies and targets single sequence repeats (SSR) or…

Identifies and targets single sequence repeats (SSR) or orthologous/taxa-specific genes for genotyping using Multiplex PCR. TipMT is a web application to search and design primers for genotyping…

MPprimer
Desktop
Web

MPprimer Multiplex Polymerase primer

A web app for designing specific, non-dimerizing primer set combinations with…

A web app for designing specific, non-dimerizing primer set combinations with constrained amplicons size for multiplex polymerase chain reaction. MPprimer employs the widely used primer design…

VisualOMP
Desktop

VisualOMP

Improves assay sensitivity by accurately predicting the secondary structure and…

Improves assay sensitivity by accurately predicting the secondary structure and locating the sites on the target that are most thermodynamically accessible.

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