A tool for superposition of multiple RNA structures. It is based on a pairwise RNA superposition algorithm called SETTER. The main idea of MultiSETTER is to build an average structure and to superpose all the input structure onto this average structure. For building the average structure we implemented a modified version of Clustal algorithm known from the sequence domain. The modification consists in replacing the sequence specific parts with their structure analogs. Thus, instead of adding sequences one by one into a resulting multiple sequence alignment as Clustal does, MultiSETTER iteratively merges RNA structures to form a resulting average structure.
Laboratory of Informatics and Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, Prague, CZ, Czech Republic; Department of Software Engineering, Faculty of Mathematics and Physics, Charles University in Prague, Malostranské nám. 25, Prague, CZ, Czech Republic