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MUSTANG specifications


Unique identifier OMICS_03642
Alternative name MUltiple STructural AligNment AlGorithm
Software type Package/Module
Interface Command line interface
Restrictions to use None
Input data Pairwise sequence alignment, pairwise structural alignment and multiple sequence alignment
Output data Multiple alignment and multiple structural superposition
Operating system Unix/Linux
Programming languages C++
Computer skills Advanced
Version 3.2.2
Stability Stable
Source code URL
Maintained Yes




No version available

Publication for MUltiple STructural AligNment AlGorithm

MUSTANG citations


Effects of genetic variants in the TSPO gene on protein structure and stability

PLoS One
PMCID: 5895031
PMID: 29641545
DOI: 10.1371/journal.pone.0195627

[…] tion, the structures were subjected to an energy minimization with the YAMBER force field as described previously []. The structures of wt and mutant TSPO proteins were superimposed and aligned using MUSTANG program implemented in the YASARA software package []. TSPO models created in this study are made available on institute homepage:( […]


Preferential selection of Arginine at the lipid water interface of TRPV1 during vertebrate evolution correlates with its snorkeling behaviour and cholesterol interaction

Sci Rep
PMCID: 5711878
PMID: 29196683
DOI: 10.1038/s41598-017-16780-w

[…] Structural alignment of the cholesterol docked closed rTRPV1 with the open conformation was done with MUSTANG multiple structural alignment algorithm included in the YASARA. The default MUSTANGPP method is a YASARA-specific extension to MUSTANG with additional Post-Processing. Starting from the initia […]


Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors

Sci Rep
PMCID: 5547150
PMID: 28785095
DOI: 10.1038/s41598-017-07620-y

[…] scores above 90% are normally associated with acceptable structures. Further validation of the AXL-D1D2 structure was obtained using the Yasara plugin of the “Multiple Structure alignment algorithm”, MUSTANG to align the AXL-D1D2 modelled structure to the Tyro3 crystal structure. MUSTANG is a structural alignment algorithm, which performs sequence alignment as well as structural superposition base […]


A shape shifting redox foldase contributes to Proteus mirabilis copper resistance

Nat Commun
PMCID: 5524982
PMID: 28722010
DOI: 10.1038/ncomms16065
call_split See protocol

[…] eo image of representative electron density for this structure is show in .Residues in all three crystal structures were numbered based on their position after the TEV cleavage site in the construct. MUSTANG-MR was used for structure alignment and RMSD calculations and PyMOL was used to create images of the structures and perform other measurements. The distance range between active sites in DsbC, […]


Exploiting sequence and stability information for directing nanobody stability engineering

Biochim Biophys Acta
PMCID: 5548252
PMID: 28642127
DOI: 10.1016/j.bbagen.2017.06.014
call_split See protocol

[…] ponding residue positions in different nanobody structures and sequences, we created a manually curated profile-to-profile alignment of a structural alignment of all nanobody chains (determined using Mustang []) with the nanobody MSA used for GSS analysis. Only one chain per entry was used for the consensus network calculation to avoid a bias towards entries with multiple chains. For multiple nano […]


Outer membrane phospholipase A’s roles in Helicobacter pylori acid adaptation

PMCID: 5469174
PMID: 28616083
DOI: 10.1186/s13099-017-0184-y

[…] All structure analyses were performed using the YASARA/WHAT IF twinset. Models and template structures were superposed using the MUSTANG pairwise protein structure aligner [] as implemented in YASARA. The OMPLA pore was visualized with different substrates with molecular surfaces shown using YASARA/WHAT IF twinset. Pore sizes w […]


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MUSTANG institution(s)
Department of Computer Science and Software Engineering, The University of Melbourne, Melbourne, VIC, Australia; Victorian Bioinformatics Consortium, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia; ARC Centre for Structural and Functional Microbial Genomics, Monash University, Melbourne, VIC USA; National ICT Australia (NICTA) Victoria Laboratory at The University of Melbourne, Melbourne, VIC, Australia; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA; Huck Institutes of the Life Sciences, Genomics, Proteomics and Bioinformatics, The Pennsylvania State University, University Park, PA, USA

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