Provides the scientific community with a simple and intuitive online primer design tool for both laboratory-scale and high-throughput projects of sequence-independent gene cloning and site-directed mutagenesis and a Tm calculator for quick queries. HTP-OligoDesigner generates cloning primer pairs by fixing the 50-end nucleotide position of each primer and varying the length of the primers from 50 end to 30 end, according to a chosen Tm calculation algorithm. The software does not check for the existence of restriction sites within the gene sequences, which would interfere in restriction enzyme/ligation methodologies.
Provides a method for researchers using CRISPR-mediated mutagenesis. indCAPS is a web application that facilitates the screening of individuals in which editing of the target has occurred. It also provides replacement for existing tools for the design of primers for dCAPS analysis capable of distinguishing known indel alleles. It was also used to design diagnostic primers to identify CRISPR-induced ahk3 null alleles.