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Provides the scientific community with a simple and intuitive online primer design tool for both laboratory-scale and high-throughput projects of sequence-independent gene cloning and site-directed mutagenesis and a Tm calculator for quick queries. HTP-OligoDesigner generates cloning primer pairs by fixing the 50-end nucleotide position of each primer and varying the length of the primers from 50 end to 30 end, according to a chosen Tm calculation algorithm. The software does not check for the existence of restriction sites within the gene sequences, which would interfere in restriction enzyme/ligation methodologies.
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