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NEMO specifications


Unique identifier OMICS_06599
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Windows
Programming languages Java
Computer skills Medium
Stability No
Maintained No


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Publication for NEMO

NEMO in publications

PMCID: 5540545
PMID: 28767705
DOI: 10.1371/journal.pone.0182398

[…] 8–16 cell stage and 0.4 μm for embryos at the morula and the blastocyst stage. series of images were processed by axiovision rel. 4.8 programme (zeiss, germany) and analysed and reconstructed using nemo software, a tool designated for gene and chromosome territory distribution analysis from 3d-fish experiments []. 3d reconstructions were created using imagej ( or icy […]

PMCID: 5045652
PMID: 27716032
DOI: 10.1186/s12860-016-0113-9

[…] an 8-bit pixel depth for each channel at a constant voxel size of 0.077 × 0.077 × 0.284 μm., segmentations and 3d measurements between objects (nucleus, genes, rna, nucleolus and ct) were done using nemo [] as described previously [], an imagej plug-in designed to interactively analyze fish 3d images and automatically measure object distances in multiple-channel experiments. the program […]

PMCID: 4987517
PMID: 27507283
DOI: 10.1038/ncomms12354

[…] positions were then acquired at higher resolution by × 63/1.4na hcx pl apo lambda blue objective (z=0.2 μm). images were processed and analysed by imagej. volumetric analysis was performed by nemo/3d-fish plugin., total rna extraction and sybr-green rt–qpcr were performed as previously described in ref. . primers used in this study are reported in . xist allele-specific expression () […]

PMCID: 4538297
PMID: 26347713
DOI: 10.3389/fendo.2015.00129

[…] images were captured at 200 nm intervals in the z-axis, using an objective fitted with a piezo nano z100 ti. analysis of nuclear position of the detected fluorescent signals was performed using nemo software (). the radial localizations of loci were then calculated in microsoft excel. three shells of equal area eroded from the center (shell 1) to the periphery (shell 3) of the nucleus […]

PMCID: 3815027
PMID: 24205066
DOI: 10.1371/journal.pone.0078005

[…] channel at a constant voxel size of 0.093×0.093×0.244 µm. typically, a stack of 30 confocal planes was acquired. segmentations and 3d measurements between objects (nucleus and ct) were done using nemo , developed from smart 3d-fish software . all objects are detected automatically by the intensity of pixels above a globally set threshold. after processing, users visually inspect image […]

NEMO institution(s)
Institut National de la Recherche Agronomique, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France

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